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S3 Cell Sorter Instruction Manual
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3.3.8 Global Preferences
Users with administrator privileges have the ability to change global settings for the instrument.
Changes to global preferences will become effective upon accepting the changes. Preferences
specific to plots will be displayed when new plots are created.
CAUTION!
These settings are global and may influence other users’ experiments.
Adjusting any of these values will change the optimal settings for the instrument.
Sheath Fluid Options
The system can use 8x or 1x sheath solution. The default concentration is 8x. The 8x sheath
fluid will mix automatically with DI water prior to running through the system. One container of
8x sheath fluid will provide up to 50 hours of run time compared to 9 hours of run time for one
container of 1x sheath solution.
If a 1x sheath solution will be used, select 1x in the sheath concentration section prior to running
the system to avoid diluting the solution.
Idle Shutdown Time
The system has the ability to shut down automatically and start the shutdown procedure after
sitting idle for a designated time period. The system has a default setting of 2 hours.
This option helps to preserve laser lifetime and prevent the system from running overnight.
Fig. 27. Global Preferences window.
Sheath can be
used at 8x or at 1x
concentration
Option to enable
mandatory cleaning
upon shutdown
or logout
Boost duration
can be increased
or decreased
Plot default options:
n
Define which plot
statistics to be
displayed and
number of digits
after the decimal
n
Choose which
axis parameter
will be displayed
and log verses
linear axis values
n
Define plot
display size,
maintain aspect
ratio of plots
n
Display and
highlight events
that are off axis
n
Display
compensation
sliders on plots
Define idle
shutdown option
and countdown time
n
Choose to prompt
user to save
unsaved FCS and
protocols
n
Define default
save file locations
Define emergency
contact information
Parameter names
can be preset to
have user-defined
names displayed
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