BIO RAD 145-1001, Instruction Manual

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54 | S3 Cell Sorter Instruction Manual 54 |
5.1.7 Auto Save
An auto save option is available as a check box on the instrument control panel. If this box is
checked (Figure 58), a prompt will appear to name and save the FCS file after an acquisition or
sort is completed.
Fig. 58. Checked auto save box.
Fig. 57. PMT control panel with name
changed on the FL1 parameter.
5.1.5 Threshold
The threshold level is set using the trigger parameter with a range of 0.1–100% (Figure 56).
The purpose of the threshold is to set an intensity level to eliminate any background noise and
debris while still allowing real events to be detected and analyzed.
5.1.6 PMT Voltages
Each parameter is collected by a photo multiplier tube (PMT). Independent voltage control is
available for each PMT. These voltages can be adjusted to bring the populations of interest
on the histogram or density plot scale (Figure 57). Custom names for each parameter can
be entered by clicking on the parameter of interest on the PMT control panel. Changing this
name will update the names on all plot axes, in the filter configuration window, and on the
compensation matrix. Refer to Section 3.3.8, Global Preferences, for information on how to set
default parameter names.