FlowStar2 LB 514
5. Analytic Splitter
43
5. Analytic Splitter
5.1 Description
The use of the
Analytic Splitter
(Figure 10) requires a configuration (ad-
mixture method) as illustrated in Figure 11.
Figure 10: Analytic splitter AS-15
E = Eluate input; FC = capillary to fraction collector; C =
capillary to measuring cell
The
AS-xx
splits off a certain fixed percentage (xx) for the radioactivity meas-
urement. This partial eluate flow is mixed with liquid scintillator by the T-
mixer (T) and passed on to the admixture cell of the HPLC Monitor. The
other share of the eluate is passed on to the fraction collector (FC).
Standard versions of the
AS-xx
have a splitting ratio of 15%, 25%, 33%
and 50%. These percentages define the share of the eluate which is
passed on to the radioactivity cell.
Due to the very thin capillaries, the system generates a backpressure of
approx. 5 bar at the input (E) of the Analytic Splitter at a flow rate of
1 ml/min (water/viscosity = 1).
The
AS-xx
operates without any problem in the flow range from 0.5 to 4
ml/min, and a possible change of the viscosity (gradient) does not affect
the splitting ratio.
IN
85% 15%
FC
Cell
E
FC
C
