5. Analytic Splitter
FlowStar2 LB 514
44
Figure 11:
Configuration with Analytic Splitter
The
AS-xx
splits off a certain fixed percentage (xx) of the eluate flow toward the
measuring cell (C). It is passed on to the T-mixer (T) and mixed with liquid scintil-
lator (LS). This cocktail is then passed on to the admixture cell (Z-xxx) of the HPLC
Monitor for radioactivity measurement. The other share of the eluate (100% minus
xx) is passed on to the fraction collector (FC).
Diameter and length of the capillaries
The outlet capillaries FC
(fraction collector)
and C
(cell; or connection to
T-mixer [T])
must not be reduced in length and increased only by a
Teflon capillary!
When using a stainless steel capillary, it must have a larger internal
diameter.
Caution: Total flow rate
2 ml/min
With a total flow rate from cocktail (liquid scinti split off elate) of < 2
ml/min, use a 60 mm long stainless steel capillary with an internal diameter
of 0.5 mm between T-piece and measuring cell.
When using longer or wider capillaries, this may lead to tailing (peak de-
formation) or double peak formation!
With low cocktail flow rates (< 2 ml/min) the distance between T-mixer (T)
and measuring cell should be fairly short.
IN
85% 15%
FC
Cell
E
FC
C
UV-Detector
Column
LS-Pump
FlowStar LB 514
2
Status
READY
Cell
Test 14C
LS-Pump
OFF
Time
125.4
20
10
0
0
2
4
6
8
CPM
C-14
I
O
Status
READY
Cell
Test 14C
LS-Pump
OFF
Time
125.4
20
10
0
0
2
4
6
8
CPM
C-14
IN
OUT
