5. Analytic Splitter
FlowStar2 LB 514
46
5.2 Installation
Do not increase or reduce the length of the capillaries which are firmly con-
nected to the
AS-xx
at the outputs FC and C. Install the connections care-
fully, since the internal diameter is only 0.1 mm.
E
(eluate supply)
0.25 mm
ID
Use the ready mounted plastic sealing cone with screw fitting. Replace-
ment in case of a possible defect or leakage in the sealing cone is quite
simple. Please note that no chips or other particles enter the
AS-xx
, for this
may block the capillaries.
The silicon hose rings located before and after the screw fitting serve
only for fixing the screw fitting.
FC
(fraction collector)
0.1 mm
ID
A 20 mm long silicon hose piece is attached to the end of this capillary.
Insert a Teflon hose (1/16") of appropriate length into the silicon hose
to pass the eluate on to the fraction collector.
C
(measuring cell)
AS-xx
T: 0.1 mm
ID; T
measuring cell: 1
mm
ID
Use the capillary spiral attached to the
AS-xx
as a connection to the T-
mixer (T). Here the split off eluate share is mixed with liquid scintillator,
transported to the radioactivity measuring cell where it is measured. Due
to the viscosity of the cocktail the in- and outlet capillaries (after the T-Mixer)
should have an internal diameter of 1 mm and be fairly short (see also page
34)
– with a total flow rate >2 ml/min. For this purpose Berthold Technolo-
gies supplies a 1 m, 1/16" capillary with an internal diameter of 1 mm to-
gether with the HPLC Monitor.
For a total flow rate of
2 ml/min
the supply line from the T-piece to the
measuring cell should have a max.
60 mm
long stainless steel capillary
with an internal diameter of
0.5 mm
.
Figure 13: Checking the splitting ratio
IN
85% 15%
FC
Cell
E
FC
C
Pump
Solvent
Measuring cylinder
Measuring cylinder
