Beckman Coulter LS 13 320, User Manual

Page: 54 / 254

PN B05577AC 2-18
Installation
Making Measurements
5
Complete the sample and run information dialog boxes with the pertinent information.
The above procedures can be greatly simplified by using either the Standard Operating Method
and/or Standard Operating Procedure features. For more on these procedures see the sample
module section.
Running a Sample
Once the performance of the instrument has been confirmed to be correct by an analysis with a
control, a sample can then be analyzed. The validity of the results from an unknown sample is based
on the good performance of the instrument, as supported by the control analysis, and by the correct
preparation of the sample in question. See
APPENDIX B,
Sample Handling
for more information on
sample dispersion and preparation. The steps to follow to analyze a sample are similar to those used
to analyze a control sample. SOPs can also be created to set up the analysis of a particular sample.
1
Run > Run Cycle
– Figure 2.9 .
2
Under
Run Cycle
select
New Sample
. If PIDS is to be used select the
Include PIDS data
check box
to activate this option. Select
Run Settings
– Figure 2.10 .
3
In the Run Settings screen ( Figure 2.16 ) choose 60 seconds for the
Run Length
. When using PIDS
select 90 seconds for the
Run Length
. Under
After each run
click on the
Compute Sizes
and
Save
File
check boxes. Select the
Model
option ( Figure 2.17 ) and choose the correct optical model. If
the optical model you need is not found in the list of optical models, you may create a new one
that will suit your particular sample (See
APPENDIX C, Optical Models ).
4
OK
.
5
Start
.