Calibrate the instrument
■
Perform spatial calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
■
Perform spectral calibration for sequencing and fragment analysis . . . . . . . . . . 97
Perform spatial calibration
The software uses images collected during spatial calibration to establish a
relationship between the signal emitted by each capillary and the position where it is
detected by the CCD camera.
Estimated run time is <5 minutes.
For all dye sets, perform a spatial calibration after you perform any of the following
actions.
• Remove or replace the capillary array
• Remove the capillary array from the detection cell block (even to adjust it)
• Move the instrument
• Move the array detection cell
Note:
Failure to perform a new spatial calibration can result in poor data quality.
1.
In the navigation pane of the Data Collection Software, select GA
Instruments
4
ga3730
4
Instrument name
4
Spatial Run Scheduler.
2.
In the Spatial Run Scheduler view,
select one of the following options
• Protocol
4
SpatialNoFill if the
capillaries contain fresh
polymer.
• Protocol
4
SpatialFill.
Note:
You do not need to fill the
capillaries each time you perform a
spatial calibration.
3.
Click Start.
4.
Evaluate the calibration. See “Evaluate the spatial calibration data“ on page 92.
8
Spatial calibration
description and
run time
When to perform a
spatial calibration
Perform spatial
calibration
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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