Task
Frequency
Check the level of buffer in the anode buffer jar.
Ensure that the overflow hole is facing toward the front of the instrument and is not
blocked.
Before each run
Replace the 1X run buffer in the buffer jar and the buffer reservoir on the
instrument, and the water in the water reservoir. Ensure that the outside of the
assemblies are dry.
When you replace the 1X run buffer, do not add fresh buffer to old buffer. Discard
old buffer, then add fresh buffer.
Every 48 hours
Check for bubbles in the pump block, lower polymer block, interconnect tube,
polymer supply tube, and channels.
Remove all bubbles using the Bubble Remove wizard.
See “Typical conditions for using maintenance wizards“ on page 117.
Daily or before each run
Check the loading-end header of the capillary array. Ensure that the capillary tips
are not crushed or damaged.
Daily or before each run
Check the level of polymer in the bottle to ensure that the volume is sufficient for
runs.
Daily or before each run
Clean the instrument surfaces.
Daily
Check for leaks around the array knob, interconnect tubing nuts, and check valve.
Daily
Weekly maintenance
Task
Frequency
Replace the polymer using the Replenish Polymer wizard or Change Polymer Type
wizard.
Weekly
Check the storage conditions of the used arrays.
Weekly
Replace reservoir septa.
Weekly
Clean the buffer jar, water, waste, and buffer reservoirs with warm water, then
rinse with distilled/deionized water.
Weekly
Flush the polymer delivery pump water trap. See “Flush the water seal trap“ on
Weekly
Chapter 9
Maintenance
Maintenance schedules
9
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3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
