Dye sets G5 and G5-RCT for fragment analysis
In fragment analysis applications using a 96-capillary array, a small peak in one color
channel that occurs at exactly the same size as a major peak in another color channel
can be incorrectly identified as an allele. This phenomena is referred to as "cross-talk"
between the different spectral channels.
The G5-RCT (Reduced Cross Talk) dye set has been optimized to minimize cross talk
peaks.
• RCT dye sets reduce potential crosstalk peaks, but also cause a decrease in overall
signal intensity.
• RCT dye sets slightly reduce signal-to-noise ratio.
• Because of the decrease in overall signal intensity, a higher dynamic range is
available when using RCT dye sets.
• The spectral calibration for an RCT dye set is specific to the capillary array on
which is it run. Do not ovveride spectral calibration when you use an RCT dye
set.
• Fragment analysis with a 96-capillary array
• Applications with a high dynamic range (where the signal intensity of large
peaks is much higher than the signal intensity of small peaks)
Fragment analysis
using a 96-
capillary array:
cross-talk peaks
Reduced cross-
talk—Dyeset G5-
RCT
When to use RCT
dye sets
Appendix D
Dye sets: G5, G5-RCT, Any4Dye, Any4Dye-HDR, and Any5Dye
Dye sets G5 and G5-RCT for fragment analysis
D
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