8.
Fill, then assemble the reservoirs.
Buffer reservoir assembly
Water and waste reservoir assemblies
1. Add 80 mL 1X run buffer to the
Buffer reservoir.
2. Assemble the reservoir assembly as
shown below.
1
2
3
4
5
6
1
Retainer
2
Septum
3
Reservoir cap
4
Reservoir
5
Heated plate base
6
Power cable
1. Add 80 mL high-quality deionized
water to each reservoir.
2. Assemble each reservoir assembly
as shown below.
1
2
3
4
5
1
Retainer
2
Septum
3
Reservoir cap
4
Reservoir
5
Plate base
9.
To prevent damage to the capillary array, inspect each reservoir for the following
conditions.
• Septa fit snugly and flush on the reservoir cap
• Rubber gasket surrounding the reservoir cap is seated correctly
1
1
Rubber gasket not seated correctly
Chapter 2
Prepare the instrument
Prepare buffer and fill the reservoirs
2
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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