100
Agilent 1290 Infinity LC System Manual and Quick Reference
5
Quick Start Guide
Data Acquisition in Method and Run Control View
Running the Method for a Single Injection
This section shows how to run a single injection of the test mix using the
conditions entered in the previous section.
Analyses on the ChemStation can be run in two modes:
• Run Method
— single injections, for example, in interactive method
development, using the parameter settings current at the time;
• Run Sequence
— automated series of injections from multiple vials, possibly
with multiple methods. For further details refer to the ChemStation
manuals.
1
Click the
Select Run Method Task
icon
.
2
If required method conditions are not currently loaded, select
Method > Load
Method
or the icon
under the menu bar to load them.
3
Place the sample vial in position 1 — this is the front position of the 10 x
2 ml vial positions on the right hand side of the sample tray.
4
Select
Run Control > Sample Info
, and enter a
Subdirectory
name (optional),
Filename
, Sample
Location
(
vial 1
),
Sample Name
and any
Comment
.
5
If the system is already equilibrated and the baseline is stable, click
Run
Method
in the
Sample Info
page to start the injection. Alternatively, click
OK
,
and when ready, click the
Start Single Sample
button above the system
diagram.
6
The injection is made, and the chromatogram appears in the
Online Plot
. The
data acquisition will stop when the
Stop Time
is reached.
The chromatogram should look similar to
on page 101 — the time
axis will be longer, however, as this was run under four times faster
conditions in the next section.
N O T E
If changes have been made to a loaded method and not yet saved, this is indicated by a
yellow asterisk on the method status icon. The injection can be made without first saving
those parameters changes. The ChemStation always stores a copy of the acquisition
parameters in the data file as ACQ.TXT to ensure the preservation of the original method
parameters.
