Agilent 1290 Infinity LC System Manual and Quick Reference
87
System Setup and Installation
4
Installing the Module
2
In the
Purge
section, set the following parameters:
•
Duration
: 6 min
•
Flow
: 10 ml/min
•
Composition B
: 50 %
Composition A
will automatically assume 50 %. Leave the On/Off button set to
Off
. Click
OK
to exit.
3
Right-click on the pump section, and select
Purge On
from the context menu.
The purge valve will now switch the flow path to the purge position, and will
simultaneously purge both channels to waste at 5 ml/min each for 6 minutes
duration. After the set duration, the purge flow will turn off, the valve will
switch the flow path back to the system, and the flow rate and composition
currently set in the method will resume. In this example, the method flow is
still set to zero. The purge settings in the
Control
page from steps 1 and 2
remain configured so when the purge process is required again it can be
started at step 3.
When the pump has been initially primed and purged with propan-2-ol, the
solvents can be exchanged for the mobile phase solvents such as water and
methanol. The purge procedure is repeated whenever a solvent is exchanged.
Immediately following the purge, the solvent in the pump has not been
degassed and so the system should be operated for at least 10 min so that the
solvent is degassed.
N O T E
Do not confuse
Purge On
with the next item
Prime On
.
