Agilent 1290 Infinity LC System Manual and Quick Reference
11
Introduction to Ultra-High Performance Liquid Chromatography
1
Theory of Using Smaller Particles in Liquid Chromatography
A chromatographic separation can be optimized based on physical parameters
of the HPLC column such as particle size, pore size, morphology of the
particles, the length and diameter of the column, the solvent velocity, and the
temperature. In addition, the thermodynamics of a separation can be
considered and the properties of the solute and the stationary and mobile
phases (percentage of organic solvent, ion strength, and pH) can be
manipulated to achieve the shortest possible retention and highest selectivity.
Figure 3
Selecting optimal conditions for HPLC
Resolution can be described as a function of three parameters:
•
column efficiency or theoretical plates (N),
•
selectivity (
),
•
retention factor (k).
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