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Agilent 1290 Infinity LC System Manual and Quick Reference
6
Appendix
Setting Up a Method using Edit Entire Method
Thermostatted Column Compartment (TCC) tab
Figure 40
Setup Method screen – Thermostatted Column Compartment tab
• Temperature
defines the temperature of the left and right-hand side column
holders which can be independently controlled or linked together by
clicking the
Combined
radio button on. When combined the settings for the
left side control both sections and this is certainly necessary when the
column is longer than 15 cm and needs to be supported by both sections.
The two sides can be operated separately when it is required to have two
columns operating at different temperatures – this can be implemented
when a column switching valve is also installed to switch between them.
Another use of separate temperature zones is when the column is operated
at a high temperature (e.g. above 60 °C) on one side and the heat exchanger
on the other side is used to cool the eluent before it enters the detector
thereby reducing noise due to thermal effects in the flow cell. Selecting the
As Detector Cell
option will automatically read the temperature from the cell
in the 1290 Infinity Detector.
