Agilent 1290 Infinity LC System Manual and Quick Reference
119
Appendix
6
Setting Up a Method using Edit Entire Method
Autosampler tab (HiP-ALS)
Figure 37
Setup Method screen –High Performance Autosampler tab
• Injection Mode
•
Injection volume
sets the volume to be injected (example 3 µl),
•
Standard injection
indicates that no external needle wash is done,
•
Injection with needle wash
is used to reduce potential carryover. This is the
recommended option and is configured in the next entry.
• Needle Wash
– if selected above.
•
Mode
determines how the outside of the needle will be rinsed: either
actively in the
Flush Port
or by dipping into a specified
Wash Vial
.
•
Time
in seconds the peristaltic pump connected to the flush port will
pump the rinse solvent. It then pumps for a further 15 s to clear the flush
port.
