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Agilent 1290 Infinity LC System Manual and Quick Reference
5
Quick Start Guide
Data Acquisition in Method and Run Control View
Setting Up the Method
This section shows how to quickly set the method conditions for an analysis
using the test mixture conditions. For a more detailed explanation of all the
available parameters see Appendix,
The default method
DEF_LC.M
has been loaded ready to prepare the new method.
Now the key parameters can be edited to create the new method. For
the example separation discussed here, set the conditions listed in
1
To quickly access the
Method
page for each module, right-click in the system
diagram for the module and select
Method...
from the context menu.
Each of the modules will be set up in this way.
2
Right-click the pump area, and select
Method...
in the context menu.
a
In the
Method
page for the
1290 Infinity Binary Pump
, enter the following
parameters:
•
Flow rate: 0.4 ml/min
•
Solvent A: Select
Water
from the compressibility drop-down list.
•
Solvent B: Select the check box to make Solvent B active.
•
%B: Initial value 40 %
•
Stop Time: 5 min
•
Max Pressure Limit: 1200 bar
b
Click the
+
sign to open the
Timetable
.
c
Add a line, select
Change Solvent Composition
, and set %B to 80 %
d
Other parameters can remain at default settings. Click
OK
to exit the
window.
The changes are sent to the pump module.
3
Right-click the autosampler area, and select
Method...
in the context menu.
a
In the
Method
page for the
1290 Infinity Autosampler
, enter the following
parameters:
•
Injection volume: 1.0 µl
•
Injection with Needle Wash
•
Mode Flush Port, Time: 6 s
