12
Agilent 1290 Infinity LC System Manual and Quick Reference
1
Introduction to Ultra-High Performance Liquid Chromatography
Theory of Using Smaller Particles in Liquid Chromatography
According to the resolution equation (
on page 12 ), the selectivity has
the biggest impact on resolution (
on page 12). This means that the
selection of appropriate mobile and stationary phase properties and
temperature is critical in achieving a successful separation.
Figure 4
Resolution equation
Figure 5
Effect of plate number, separation factor and retention factor on R
No matter whether the rapid resolution separation method is being newly
developed or simply transferred from an existing conventional method, it is
clearly beneficial to have a wide choice of stationary phase chemistries
available in a range of column formats.
