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Agilent 1290 Infinity LC System Manual and Quick Reference

Agilent Technologies

3
Optimization of the Agilent 1290 Infinity
LC System

Delay Volume and Extra-Column Volume

Delay Volume

Extra-Column Volume

How to Configure the Optimum Delay Volume

How to Achieve Higher Injection Volumes

How to Achieve High Throughput

How to Achieve Higher Resolution

How to Achieve Higher Sensitivity

How to Achieve Lowest Carry Over

How to Prevent Column Blockages

This chapter considers how to apply the theory and use the features of the LC
system to develop optimized separations.

Summary of Contents for 1290 Infinity LC System

Page 1: ...Agilent Technologies Agilent 1290 Infinity LC System System Manual and Quick Reference...

Page 2: ...terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied o...

Page 3: ...atures of the 1290 Infinity LC System 3 Optimization of the Agilent 1290 Infinity LC System This chapter considers how to apply the theory and use the features of the LC system to develop optimized se...

Page 4: ...Components 25 3 Optimization of the Agilent 1290 Infinity LC System 39 Delay Volume and Extra Column Volume 40 How to Configure the Optimum Delay Volume 42 How to Achieve Higher Injection Volumes 51...

Page 5: ...290 Infinity LC System Manual and Quick Reference 5 Contents 6 Appendix 109 Safety Information 110 Solvent Information 113 Agilent Technologies on Internet 114 Setting Up a Method using Edit Entire Me...

Page 6: ...6 Agilent 1290 Infinity LC System Manual and Quick Reference Contents...

Page 7: ...on to Ultra High Performance Liquid Chromatography Theory of Using Smaller Particles in Liquid Chromatography 8 Benefits of Columns Packed With Sub 2 micron Particles 14 Frictional Heating 18 This cha...

Page 8: ...two micron particles offer the opportunity to dramatically reduce analysis time by increasing the flow rate without losing separation performance 2 Higher Resolution Long columns with sub two micron p...

Page 9: ...A term relates to eddy diffusion or multiple flow paths through the column B relates to molecular diffusion along the column axis longitudinal C relates to mass transfer of the analyte between the mob...

Page 10: ...ificing resolution and hence the analysis time is also reduced by a factor of two to three The increased efficiency is derived to a large extent from the reduction in multiple flow paths as a result o...

Page 11: ...red and the properties of the solute and the stationary and mobile phases percentage of organic solvent ion strength and pH can be manipulated to achieve the shortest possible retention and highest se...

Page 12: ...This means that the selection of appropriate mobile and stationary phase properties and temperature is critical in achieving a successful separation Figure 4 Resolution equation Figure 5 Effect of pl...

Page 13: ...selecting the appropriate column length and diameter and operation with longer STM columns has become more accessible than ever before Many laboratories perform an extensive screening process to selec...

Page 14: ...columns This calculator is available on the Agilent Technologies web site along with a method translator calculator www chem agilent com The results are presented graphically and in tabular form Shor...

Page 15: ...t 1290 Infinity LC System Manual and Quick Reference 15 Introduction to Ultra High Performance Liquid Chromatography 1 Benefits of Columns Packed With Sub 2 micron Particles Figure 6 Cost savings calc...

Page 16: ...iquid Chromatography Benefits of Columns Packed With Sub 2 micron Particles Figure 7 Relation between particle size efficiency and analysis time 8dajbc AZc i bb 8dajbc 8dajbc 8dajbc X ZcXn X ZcXn X Zc...

Page 17: ...s or proteomics studies Also applications such as impurity profiling can benefit from higher separation power Even the LC MS analysis of drugs in biological fluids can benefit from the higher peak cap...

Page 18: ...micron columns still deliver superior efficiencies compared with the respective 2 1 mm inner diameter columns An example of a gradient method transfer to a 2 1 mm i d STM column in which the separati...

Page 19: ...l offers benefits of increased efficiency higher resolution and faster separations The Agilent 1290 Infinity LC System and RRHD columns increase the separation space available and enable more of these...

Page 20: ...20 Agilent 1290 Infinity LC System Manual and Quick Reference 1 Introduction to Ultra High Performance Liquid Chromatography Frictional Heating...

Page 21: ...and Quick Reference Agilent Technologies 2 The Agilent 1290 Infinity LC System Product Description New Features of the Agilent 1290 Infinity LC System 22 System Components 25 This chapter discusses th...

Page 22: ...mm internal diameter and all types of column packing material Pressure range up to 1200 bar 17400 psi allows for fast chromatography on short columns high resolution on long columns using sub two micr...

Page 23: ...tes that the system can use This operating envelope can be described as the power range flow x pressure of the instrument and is most easily understood in graphical form Figure 10 on page 23 As can be...

Page 24: ...low flow applications such as required by some MS systems or up to 5 mm id typically 4 6 mm for more traditional LC or increased loadability The flow rate range also supports the latest research find...

Page 25: ...tand the workload but also to actively transfer heat from the seals microfluidic heat exchangers and the Jet Weaver a microfluidic mixing device The pump can deliver flow in the range of 0 05 5 mL min...

Page 26: ...r step The movement of the pistons is under intelligent control with a feedback loop to ensure that this active damping of pressure pulsation results in a ripple free flow The piston drive tunes itsel...

Page 27: ...available for use to extend the life of the pump seals A solvent selection valve allows binary mixtures isocratic or gradient to be formed from one of two solvents per channel Binary gradients are cre...

Page 28: ...oise in these demanding applications a 380 L Jet Weaver is also available For MS detection it is often possible to work without the Jet Weaver and attain sufficient mixing using only the base 10 l vol...

Page 29: ...e as that in the 1290 Infininty Binary Pump and therefore has the same performance values and technical details It can also be equipped with an active seal wash to extend the lifetime of the pump seal...

Page 30: ...Valve functionalities Figure 14 Standard application Figure 15 Extra mixing volume setup Figure 16 Automatic purge function Figure 17 Backflushing the in line filter Pump Filter Sampler Waste Agilent...

Page 31: ...is while the extra mixing volume setup 2 is applied for any kind of baseline criticial application where the mixing performance and the dependant UV baseline can be significantly enhanced by the use o...

Page 32: ...raulic volume of the flow path is suited to faster gradients and the ability to use overlapped injections and automatic delay volume reduction ADVR contribute to faster cycle times and even faster gra...

Page 33: ...sampler and so may be preferred in some fast gradient high throughput situations The trade off is that the flexibility of variable volume injection is turned off and some sample is wasted in flushing...

Page 34: ...a 1 6 l low dispersion heat exchanger and each valve kit contains additional low dispersion heat exchangers for each column The low dispersion heat exchangers up to 4 can be mounted flexibly inside th...

Page 35: ...ional columns available for different applications Thus the column to be used becomes a simple method parameter This requires two 8 position 9 port valve heads one each in two of the TCCs Clustered TC...

Page 36: ...lications The Agilent Max Light cartridge cell dramatically increases the light transmission by utilizing the principle of total internal reflection along a non coated fused silica capillary achieving...

Page 37: ...is generally operated in the middle of the range but for optimization can be closed down to 1nm for high spectral resolution rarely required in liquid phase UV spectra or opened up to 8 nm for maximu...

Page 38: ...38 Agilent 1290 Infinity LC System Manual and Quick Reference 2 The Agilent 1290 Infinity LC System Product Description System Components...

Page 39: ...olumn Volume 41 How to Configure the Optimum Delay Volume 42 How to Achieve Higher Injection Volumes 51 How to Achieve High Throughput 53 How to Achieve Higher Resolution 56 How to Achieve Higher Sens...

Page 40: ...re significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small d...

Page 41: ...autosampler Extra Column Volume Extra column volume is a source of peak dispersion that will reduce the resolution of the separation and so should be minimized Smaller diameter columns require propor...

Page 42: ...me of about 0 2 min which is generally acceptable with gradient times of two to three minutes see Table 6 on page 43 It is often useful to consider the flow rate in terms of column volumes and it can...

Page 43: ...0 Infinity Quaternary LC system configurations System Configurations1 1 added 5 l to allow for connections in system configurations Delay Volume l Quaternary Pump Fixed Loop Autosampler MS only 360 Qu...

Page 44: ...pillaries back to the injection valve see Figure 18 on page 32 To make an injection the valve switches from mainpass to bypass so that the metering device can draw the sample into the needle capillary...

Page 45: ...but may be a factor to be considered in a method transfer With a flush out factor of 5 and an injection volume of 10 l the autosampler will allow 50 l to pass through before switching to bypass which...

Page 46: ...rectly from the purge valve Ensure that the Jet Weaver has been flushed with solvent containing no buffers or other additives before disconnecting it Sometimes it may be advisable to increase the dela...

Page 47: ...it is still possible to install an optional 380 L Jet Weaver mixer for baseline critical applications such as TFA applications The optional Jet Weaver mixer has a different housing which is adapted t...

Page 48: ...he hex screws that fix the Jet Weaver to the pump housing 3 Install the new Jet Weaver NOTE The Jet Weaver has a front and a rear side with different internal volumes 35 100 l that are optimized for a...

Page 49: ...lling the V380 Jet Weaver in the 1290 Infinity Quaternary Pump 1 Open the screw of the Jet Weaver metal lid 2 Remove the metal lid by lifting it up 1 and pulling it out of the front panel 2 3 Insert t...

Page 50: ...ick Reference 3 Optimization of the Agilent 1290 Infinity LC System How to Configure the Optimum Delay Volume 5 Connect the inlet capillary of the Jet Weaver to port 2 of the Multi Purpose Valve Conne...

Page 51: ...nt the same or weaker than the starting gradient composition This has a bearing on whether or by how much the injection volume can be increased and the user should check for signs of increased dispers...

Page 52: ...tion Volumes One way to achieve larger injections is to use a trapping column selected by a switching valve to capture and concentrate the injection before switching it i e injecting it onto an analyt...

Page 53: ...iency available in a short column If the methods involve isocratic separation this allows for the fastest cycle times because no column equilibration is required between runs However most often gradie...

Page 54: ...o do this during column equilibration when the autosampler has seen the starting conditions of the next gradient run The column equilibration step can be a significant part of the cycle time Typically...

Page 55: ...Agilent 1290 Infinity LC System Manual and Quick Reference 55 Optimization of the Agilent 1290 Infinity LC System 3 How to Achieve High Throughput Figure 22 Alternating Column Regeneration...

Page 56: ...ks is described by the resolution equation where Rs resolution N plate count measure of column efficiency selectivity between two peaks k2 retention factor of second peak formerly called capacity fact...

Page 57: ...50 mm length Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1 4 What is achievable depends on the viscosity of the mobile p...

Page 58: ...resolution can be increased by having a shallower gradient 2 to 5 min change is a guideline higher flow rate and a smaller volume column This equation also shows how to speed up an existing gradient i...

Page 59: ...applications but when using TFA in the mobile phase or other situations requiring more mixing the larger volume side of the Jet Weaver mixer should be used to minimize mixing noise Columns Sensitivit...

Page 60: ...the peak dispersion volume calculated from peak width x flow rate should be greater than about 2 l for this cell for example 0 02 min x 200 l min 4 l The Max Light high sensitivity cell has a path le...

Page 61: ...d If possible the range where solvents absorb strongly should be avoided below 220 nm for methanol below 210 nm for acetonitrile For example in Figure 23 on page 62 anisic acid has a suitable absorban...

Page 62: ...efault reference is 360 nm with a bandwidth of 100 nm A wide bandwidth is used because this reduces the noise in the reference signal from statistical theory the error i e noise in this case is reduce...

Page 63: ...dth of 4 nm It follows that the minimum optical resolution will be 4 nm and therefore the diode array or digital bandwidth should be set to 4 nm or greater For optimum sensitivity the 8 nm setting wil...

Page 64: ...ak width setting response time and data rate in the detector are all linked The available settings are shown in Table 8 on page 65 It is important to set this correctly for optimum sensitivity and to...

Page 65: ...a collection rate such that it collects enough data points over the narrowest peaks and it is aiming for 15 to 25 points across a peak The 1290 Infinity DAD can collect at a maximum 160 Hz if required...

Page 66: ...ounds liable to stick in the system The following functions of the autosampler can be used to minimize carryover Internal needle wash External needle wash Needle seat backflush Injection valve cleanin...

Page 67: ...dule The flush port and its solvent delivery pump and tubing should be regularly flushed to ensure the lowest carryover For example before using the system each day prime the flush pump for three minu...

Page 68: ...small pore size to prevent packing material being swept out This immediately brings increased risk of blocking these frits with particulates from the sample mobile phase and from the instrument itself...

Page 69: ...inner diameter column and 0 4 ml min for 4 6 mm inner diameter Increase the flow rate to the desired flow over 5 minutes 11 Once the pressure has stabilized attach the column to the detector 12 Equili...

Page 70: ...70 Agilent 1290 Infinity LC System Manual and Quick Reference 3 Optimization of the Agilent 1290 Infinity LC System How to Prevent Column Blockages...

Page 71: ...Module 73 Optimizing the Stack Configuration Binary LC System 73 Optimizing the Stack Configuration Quaternary LC System 78 Priming the Pump 83 Purging the Pump 85 Flow Connections Between Modules 88...

Page 72: ...reases productivity through automatic notification of maintenance and service needs with the use of advanced counters This allows a problem to be fixed before it impacts results The software includes...

Page 73: ...tions maintenance and parts Optimizing the Stack Configuration Binary LC System One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1290 Infinity Binary LC Syst...

Page 74: ...al and Quick Reference 4 System Setup and Installation Installing the Module Figure 25 Recommended stack configuration for 1290 Infinity with binary pump front view 9ZiZXidg 6jidhVbeaZg Ejbe HdakZci X...

Page 75: ...erence 75 System Setup and Installation 4 Installing the Module Figure 26 Recommended stack configuration 1290 Infinity with binary pump rear view 68 EdlZg A6C id A8 8 ZbHiVi dc 86C 7jh XVWaZ 6cVad YZ...

Page 76: ...pump and thermostat at the bottom of each stack and avoids high stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is req...

Page 77: ...m Setup and Installation 4 Installing the Module Figure 28 Recommended two stack configuration for 1290 Infinity with binary pump rear view 86C 7jh XVWaZ id chiVci E adi 6cVad YZiZXidg h cVa dei dcVa...

Page 78: ...tion Ensure optimum performance by installing the modules of the Agilent 1290 Infinity Quaternary LC System in the following configuration see Figure 29 on page 79 and Figure 30 on page 80 This config...

Page 79: ...d Quick Reference 79 System Setup and Installation 4 Installing the Module Figure 29 Recommended stack configuration for 1290 Infinity with quaternary pump front view Instant Pilot Solvent cabinet Det...

Page 80: ...nce 4 System Setup and Installation Installing the Module Figure 30 Recommended stack configuration for 1290 Infinity with quaternary pump rear view CAN Bus cable Analog detector signal optional CAN B...

Page 81: ...p and thermostat at the bottom of each stack and avoids high stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is requir...

Page 82: ...Setup and Installation Installing the Module Figure 32 Recommended two stack configuration for 1290 Infinity with quaternary pump rear view LAN to control software CAN Bus cable to Instant Pilot Analo...

Page 83: ...hold health and safety risks When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material...

Page 84: ...for each of the remaining empty solvent tubes a Disconnect the empty tubing at the solvent select valve Attach the priming syringe with threaded adaptor to the tubing b Slowly draw solvent through the...

Page 85: ...th a co miscible solvent isopropanol is often a good choice check with a solvent miscibility table The inlet tubes to the pump should already be full of solvent If the inlet tubes are partially or tot...

Page 86: ...4 System Setup and Installation Installing the Module 1 To access the setup page for controlling the purge valve right click on the pump section and select Control from the context menu Alternatively...

Page 87: ...ill turn off the valve will switch the flow path back to the system and the flow rate and composition currently set in the method will resume In this example the method flow is still set to zero The p...

Page 88: ...eel flexible capillary from port 6 on the autosampler injection valve to the thermostatted column compartment Connect the capillary direct to the low dispersion volume heat exchanger for minimal delay...

Page 89: ...Plot 93 Purging the Pump 95 Data Acquisition in Method and Run Control View 96 Method Parameters for Test Mixture and ZORBAX RRHD Column 96 Setting Up the Method 98 Running the Method for a Single In...

Page 90: ...bout method parameters This example demonstrates setting up and running an analysis using the column and checkout sample provided with the Agilent 1290 Infinity LC system The example refers to menus a...

Page 91: ...lect Start All Programs Agilent Technologies OpenLAB OpenLAB Control Panel Select the relevant instrument in the navigation pane under Instruments and click Launch online The ChemStation software open...

Page 92: ...1 Go to Method and Run Control view of the ChemStation 2 On the menu bar select Method New Method and select DEF_LC M from the context menu Alternatively you can use the Load Method icon under the me...

Page 93: ...uide 5 Preparing the system Configuring the Online Plot 1 If the Online Plot window is not visible Click View Online Signals Signal Window 1 to display the window 2 To configure the desired signal s i...

Page 94: ...ible This is useful for checking the baseline and looking at previous injections The X and Y axis scales can be adjusted directly with the up down buttons on each axis The Adjust button in the Online...

Page 95: ...e for a few hours or more air may have diffused into the solvent lines and purging is recommended The solvent reservoirs are refilled and the pump requires purging to fill the system with fresh solven...

Page 96: ...xture and ZORBAX RRHD Column The 1290 Infinity LC System is supplied with a ZORBAX RRHD Eclipse Plus C18 1 8 m 2 1 mm x 50 mm column and a test mixture containing phenones for use with this example pr...

Page 97: ...Setting Pump Solvent A Water Solvent B Acetonitrile Flow rate 0 4 ml min Initial Composition 60 A 40 B Gradient Timetable At 4 minutes 20 A 80 B Stop Time 5 minutes Autosampler Injection 1 l Needle w...

Page 98: ...for the module and select Method from the context menu Each of the modules will be set up in this way 2 Right click the pump area and select Method in the context menu a In the Method page for the 129...

Page 99: ...area and select Method in the context menu a In the Method page for the 1290 Infinity DAD enter the following parameters Use Signal Turn all signals except Signal A off by clearing the check boxes Sig...

Page 100: ...ions on the right hand side of the sample tray 4 Select Run Control Sample Info and enter a Subdirectory name optional Filename Sample Location vial 1 Sample Name and any Comment 5 If the system is al...

Page 101: ...rate 1 6 ml min Gradient Change the gradient so that the gradient slope is unchanged in terms of volume compared to the first run Flow has been increased by 4x therefore reduce the gradient time by 4x...

Page 102: ...n further experience with running the system by trying to optimize the method further Some changes that may help are Reduce the concentration of the sample by diluting 1 10 Increase the range of the g...

Page 103: ...ta to give quantitative and qualitative results This section looks briefly at integration and reports in data analysis so that the separations generated earlier in this chapter can be integrated and p...

Page 104: ...Data Analysis in the bottom left of the screen see Figure 34 on page 103 3 In the Navigation Panel find the data directory containing the data files All the single injection data are represented as a...

Page 105: ...w 2 Click the Set Integration Events Table icon to open the table as shown 3 Set Baseline Correction to Advanced for gradient runs 4 Set Slope Sensitivity to 50 Higher numbers will integrate steeper p...

Page 106: ...106 Agilent 1290 Infinity LC System Manual and Quick Reference 5 Quick Start Guide Data Analysis 8 Exit from the Events Table using the green tick icon see figure below...

Page 107: ...nt 1290 Infinity LC System Manual and Quick Reference 107 Quick Start Guide 5 Data Analysis Specify the Report 1 On the menu bar click Report Specify Report to display the window shown in the figure b...

Page 108: ...is actually a directory The report file can be viewed directly in ChemStation or it can be found in the directory using the normal Windows File Explorer 4 Save the method once again to ensure the rep...

Page 109: ...tion 110 Solvent Information 113 Agilent Technologies on Internet 114 Setting Up a Method using Edit Entire Method 115 Method Information 117 Instrument Acquisition 118 Data Analysis 133 Run Time Chec...

Page 110: ...thing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed...

Page 111: ...hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do...

Page 112: ...icates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user s...

Page 113: ...concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are les...

Page 114: ...Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemi...

Page 115: ...ough a series of screens which each focus on one module or function These screens are accessed by clicking an icon on the graphical user interface GUI or through the menu bar with its drop down menus...

Page 116: ...or parameters pump parameters oven parameters detector parameters and instrument curves Data Analysis comprises signal details integration parameters and reporting parameters Run Time Checklist compri...

Page 117: ...n can also be directly accessed through the menu Method Method Information or by right clicking on the graphical user interface This box allows information about the method to be entered This informat...

Page 118: ...Program Binary Pump BinPump Thermostatted Column Compartment TCC Diode array Detector DAD Instrument Curves To move between the tabs click on the tab name at the top of the screen When parameter chan...

Page 119: ...on indicates that no external needle wash is done Injection with needle wash is used to reduce potential carryover This is the recommended option and is configured in the next entry Needle Wash if sel...

Page 120: ...ace the tip of the needle 3 mm above the bottom of the vial Equilibration time is a delay time between drawing the sample and moving the needle Sample flush out factor determines how long the autosamp...

Page 121: ...the injection has taken place either after the sample has flushed out of the injector or at some specified later time in the run The injector will then aspirate the next sample in readiness for the ne...

Page 122: ...am tab This allows specialized injection procedures to be constructed which involve the manipulation of aliquots from multiple vials as for instance in pre column derivatization Reagent chemicals are...

Page 123: ...ed Solvents defines the mobile phases that are available and the percentage proportions pumped on the two channels A and B On each channel a drop down box allows the selection of a solvent from a list...

Page 124: ...meter box to reveal the entry box for the value If timetable entries are made out of logical sequence the entries are automatically re ordered into time order Timetable lines can be edited directly an...

Page 125: ...revented It allows time for the system to re equilibrate after a gradient run For an isocratic method it can be set to Off For a gradient method the value can be determined experimentally by observing...

Page 126: ...sary when the column is longer than 15 cm and needs to be supported by both sections The two sides can be operated separately when it is required to have two columns operating at different temperature...

Page 127: ...s controlled to 0 15 C down to 10 C below ambient although it should be noted that there are very few applications operating below 12 15 C Avoid using the TCC at such low temperature that condensation...

Page 128: ...signal is required that box is checked and defined Wavelength sets the central wavelength nm of the signal Bandwidth sets the width nm of the signal Reference Wavelength sets the central wavelength n...

Page 129: ...convenience of setting Stop Times to As Pump Timetable operates in the same way as other modules that is add a line select the function to be changed and enter the new values for that function The ch...

Page 130: ...ing of 2 nm is a good choice for most applications Threshold determines that spectra are not stored for peak heights below this value mAU Advanced Analog Output The 1290 Infinity detector has one anal...

Page 131: ...nts on the spectrum to zero and hence also zeroes the baseline signal Prerun is selected to balance just before the start of the run which is the normal situation Sometimes Postrun is alternatively se...

Page 132: ...es tab allows monitored data streams other than detector signals to be stored with the data by checking the relevant box These are primarily used for diagnostic purposes They are Pump Pressure Flow A...

Page 133: ...n the lower part of the screen You can select any or all of the acquired detector signals for processing If no signals are selected the table is empty in this situation ChemStation will default to pro...

Page 134: ...134 Agilent 1290 Infinity LC System Manual and Quick Reference 6 Appendix Setting Up a Method using Edit Entire Method Figure 43 Signal details...

Page 135: ...Events in the context menu In the Data Analysis view it can be accessed through the menu Integration Integration Events or the Edit Integration Events task icon Figure 44 Edit Integration Events scree...

Page 136: ...ntegrator to distinguish between noise and very small peaks Area Reject Height Reject values that control rejection from the results of peaks whose area or height falls below these values Integration...

Page 137: ...Appendix 6 Setting Up a Method using Edit Entire Method Figure 45 Specify Report Screen To setup a simple area report with Classic Reporting which prints to the printer and to a PDF file enter the fol...

Page 138: ...ive results sorted by Signal Add Chromatogram Output Checked Chromatogram Output Portrait Size Time axis 100 of page Response axis 40 of page Destination Printer Checked Screen Unchecked File Checked...

Page 139: ...d Quick Reference 139 Appendix 6 Setting Up a Method using Edit Entire Method Instrument Curves Figure 46 Instrument Curves screen The Instrument Curves checkboxes allow these recorded parameters to b...

Page 140: ...offers an opportunity to link macro commands or programs into the work flow at various points In most cases the Data Acquisition and Standard Data Analysis check boxes are checked If no data analysis...

Page 141: ...in the usual configuration as the software always saves the method in the data file all versions since B 02 01 Only if the ChemStation has been configured such that Unique Sequence Folder Creation is...

Page 142: ...s 103 DEF_LC M 115 delay volume description 40 example 40 detector achieving higher sensitivity 60 Diode Array Detector description 36 E extra column volume description 41 F flow cell 113 Max Light ca...

Page 143: ...guide introduction 90 R report specification 107 resolution 12 17 Optimization 56 response time 64 retention factor 12 Run Time Checklist 116 S safety class I 110 safety general information 110 symbo...

Page 144: ...G4220 90301 www agilent com Agilent Technologies In This Book This manual contains technical reference information about the Agilent 1290 Infinity LC System The manual describes the following introdu...

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