Agilent 1290 Infinity LC System Manual and Quick Reference
69
Optimization of the Agilent 1290 Infinity LC System
3
How to Prevent Column Blockages
9
Purge the pumps (the connections up to the column) of any buffer
containing mobile phases and flush through 5 ml of solvent before attaching
the column to the instrument.
10
Flush the column with compatible mobile phase starting slowly at
0.1 ml/min for a 2.1 mm inner diameter column, 0.2 ml/min for a 3.0 mm
inner diameter column, and 0.4 ml/min for 4.6 mm inner diameter. Increase
the flow rate to the desired flow over 5 minutes.
11
Once the pressure has stabilized, attach the column to the detector.
12
Equilibrate the column and detector with 10 column volumes of the mobile
phase prior to use (see
on page 44 for column volumes).
13
Avoid over-pressure. Check the pressure range of your gradient before
starting any sequence.
14
If samples are dissolved in high organic content consider using an injection
program to add a plug of weaker solvent before and after the sample aliquot
to reduce the risk of precipitation in high concentration buffer mobile
phase.
