Agilent 1290 Infinity LC System Manual and Quick Reference
17
Introduction to Ultra-High Performance Liquid Chromatography
1
Benefits of Columns Packed With Sub-2-micron Particles
Higher Resolution
Long columns packed with smaller particles result in higher efficiency and
higher resolution. This is important for analysis of complex samples from
metabolomics or proteomics studies. Also, applications such as impurity
profiling can benefit from higher separation power. Even the LC/MS analysis
of drugs in biological fluids can benefit from the higher peak capacity, because
of the reduced interference from ion suppression. In general, higher
separation power provides more confidence in the analytical results.
Figure 8
Peak capacities of more than 700 can be achieved using a ZORBAX RRHT
SB-C18 column (2.1 x 150 mm, 1.8 µm) to analyze a tryptic digest of BSA
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