Agilent 1290 Infinity LC System Manual and Quick Reference
97
Quick Start Guide
5
Data Acquisition in Method and Run Control View
Table 9
Method parameters for first separation run
Module
Parameter
Setting
Pump
Solvent A
Water
Solvent B
Acetonitrile
Flow rate
0.4 ml/min
Initial Composition
60 % A, 40 % B
Gradient Timetable
At 4 minutes 20 % A, 80 % B
Stop Time
5 minutes
Autosampler
Injection
1 µl
Needle wash
Flush port, 6 s
TCC
Column
ZORBAX Eclipse Plus C18
1.8 µm, 2.1 mm x 50 mm i.d.
Temperature
40 °C
Detector
Signal A
250 nm, bw 100 nm, ref
360 nm, bw 100 nm
Peak width
0.025 min (10 Hz)
Store Spectra
All
