Agilent 1290 Infinity LC System Manual and Quick Reference
99
Quick Start Guide
5
Data Acquisition in Method and Run Control View
b
Other parameters can remain at default settings. Click
OK
to exit the
window.
The changes are sent to the autosampler module.
4
Right-click the Thermostatted Column Compartment (TCC) area, and select
Method...
in the context menu.
a
In the
Method
page for the
1290 Infinity TCC
, enter the following
parameters:
•
Left Temperature 40 °C
•
Right Temperature Combined
b
Other parameters can remain at default settings. Click
OK
to exit the
window.
The changes are sent to the TCC module.
5
Right-click the Diode-Array Detector area, and select
Method...
in the
context menu.
a
In the
Method
page for the
1290 Infinity DAD
, enter the following
parameters:
•
Use Signal
: Turn all signals except
Signal A
off by clearing the check
boxes.
•
Signal A: 250 nm, bw 100 nm, ref 360 nm, bw 100 nm
•
Peak width: 0.012 min (0.25 s Response, 20 Hz)
b
In the
Advanced
section, set
Spectrum Store
to
All
.
c
Other parameters can remain at default settings. Click
OK
to exit the
window.
The changes are sent to the DAD module.
6
All the required module parameters have now been entered. Select
Method >
Save Method As...
to save the method with a new name.
The ChemStation will not allow the method to be saved as
DEF_LC.M
, so that
the default method template is not altered.
7
Allow the system to equilibrate for at least 10 min, and check that the
baseline in the
Online Plot
is stable before starting the analysis.
