84
Agilent 1290 Infinity LC System Manual and Quick Reference
4
System Setup and Installation
Installing the Module
1
Prepare channel A and pump head A for priming:
a
Part fill each solvent reservoir with ca. 150 ml of HPLC grade propan-2-ol
for priming the pump and place the sintered glass filter ends of the
solvent tubing in the reservoirs.
b
Disconnect the tubing entering the inlet check valve of pump head A.
This is the outlet tubing from vacuum degasser channel A.
c
Attach the priming syringe with threaded adaptor to the tubing.
d
Slowly draw solvent through the tubing until all bubbles have been
removed.
e
Quickly remove the syringe and adaptor from the tubing and attach the
tubing to the inlet check valve on pump head A.
2
Repeat previous step for solvent channel B and pump head B.
3
If a solvent selection valve (SSV) is installed in the pump, carry out the
following steps for each of the remaining empty solvent tubes:
a
Disconnect the empty tubing at the solvent select valve. Attach the
priming syringe with threaded adaptor to the tubing.
b
Slowly draw solvent through the tubing until all bubbles have been
removed.
c
Quickly remove the syringe and adaptor from the tubing and re-attach
the tubing to the solvent selection valve.
4
In the ChemStation software, right-click on the pump section of the system
diagram and then select
Prime On
from the context menu (do not confuse it
with the
Purge On
command). Other controllers have a similar function.
The purge valve will now switch flow paths A and B to the waste position and
will simultaneously prime both channels. The module draws solvent, at high
speed with all four pump-drives simultaneously, and dispenses it to the waste
position of the automatic purge valve. This is repeated 20 times and then the
priming process ends. The purge valve switches the flow path back to the
system.
It is recommended that each channel is now purged with 30 ml of propan-2-ol.
Follow the procedure
