Agilent 1290 Infinity LC System Manual and Quick Reference
43
Optimization of the Agilent 1290 Infinity LC System
3
How to Configure the Optimum Delay Volume
Table 4
Delay volumes of 1290 Infinity Binary LC system configurations
System Configurations
1
1
added 5 µl to allow for connections in system configurations
Delay Volume (µl)
Binary Pump + Fixed Loop Autosampler (MS only)
20
Binary Pump + Jet Fixed Loop
55
Binary Pump + standard Autosampler (MS only)
90
Binary Pump + Jet Autosampler
125
Table 5
Delay volumes of 1290 Infinity Quaternary LC system configurations
System Configurations
1
1
added 5 µl to allow for connections in system configurations
Delay Volume (µl)
Quaternary Pump + Fixed Loop Autosampler (MS only)
360
Quaternary Pump + standard Autosampler (MS only)
430
Quaternary Pump + V380 Jet Fixed Loop
510
Quaternary Pump + V380 Jet Autosampler
580
Table 6
System delay times for gradient to reach head of column
Flow
Rate
(ml/min)
System Delay Volume (microliters)
20
55
90
125
360
395
430
465
Delay Times (minutes)
0.2
0.10
0.28
0.43
0.60
1.80
2.15
2.55
2.90
0.4
0.05
0.14
0.21
0.30
0.90
1.08
1.28
1.45
0.6
0.03
0.09
0.14
0.20
0.60
0.72
0.85
0.97
0.8
0.03
0.07
0.11
0.15
0.45
0.54
0.64
0.73
1.0
0.02
0.06
0.09
0.12
0.36
0.43
0.51
0.58
