Agilent 1290 Infinity LC System Manual and Quick Reference
101
Quick Start Guide
5
Data Acquisition in Method and Run Control View
Running the method faster
The first exercise ran at a pressure achievable in a standard system. Now the
flow rate is increased and the gradient adjusted for a faster separation.
1
Edit the method conditions in the same way as in the previous section to
make the following changes:
•
Flow rate: 1.6 ml/min
•
Gradient: Change the gradient so that the gradient slope is unchanged in
terms of volume compared to the first run. Flow has been increased by
4x therefore reduce the gradient time by 4x; set the gradient time to
1 min.
•
Stop Time: 1.25 min
2
Save the method with a new name.
3
Ensure that in
Sample Info
a new filename is used.
4
When the baseline is stable and equilibrated, start the run using the
Start
Single Sample
button.
The chromatogram should look similar to the one shown below, with a run
time of about 1 min.
Figure 33
Example chromatogram of the phenones test mixture using Quick Start
conditions
