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Axioskop 40
OPERATION
Axioskop 40 FL
Illumination and contrast procedures
Carl Zeiss
B 40-810 e 12/01
3-21
3.5
Illumination and contrast procedures
3.5.1
Setting transmitted-light bright field according to KÖHLER
(1)
General principle
Transmitted-light bright field microscopy is the most commonly used of all the optical techniques, since
it makes for fast and easy viewing of high-contrast or stained specimens (e.g. blood smears).
For as true-to-object imaging as possible, indirect ray bundles, i.e. ray bundles diffracted and scattered
on the specimen details, are of major importance in addition to so-called direct ray bundles. The greater
the portion of indirect rays (aperture), the more realistic the microscope image will be, according to
ABBE.
To make full use of the entire optical performance of the microscope - especially of the objective, the
condenser, the luminous-field diaphragm and the aperture diaphragm should be set in accordance with
the rules for KÖHLER illumination. These basic rules of microscope setting are described in detail in
Section 3.5.1 (3) "Transmitted-light bright field settings according to KÖHLER on the Axioskop 40 and
Axioskop 40 FL".
(2)
Transmitted-light bright field equipment on the Axioskop 40 and Axioskop 40 FL
Each Axioskop 40 microscope permits performance of the transmitted-light bright field technique.
Either the 0.9/1.25 H condenser or the 0.9 universal condenser can be used.
(3)
Setting transmitted-light bright field according to KÖHLER on the Axioskop 40 and
Axioskop 40 FL
−
The Axioskop 40 has been set up as described in Chapter 2.
−
The Axioskop 40 has been switched on.
•
Set image brightness via voltage control (3-18/
1
) on the microscope stand.
•
Place a high-contrast specimen on the specimen holder of the mechanical stage.
•
Swivel in front lens of universal condenser (for objectives
≥
10x) and use drive for height adjustment
(3-19/
2
) to move the condenser to the upper stop. The stop must be set in such a manner that the
specimen is not touched by the condenser (for setting of condenser stop, see Section 3.5.1
(4)).
•
Depending on the condenser used:
Switch slider (3-19/
3
) of the 0.9/1.25 H condenser (3-19/
4
) to the middle position for bright field or
the turret disk of the 0.9 universal condenser (if equipped with a turret disk) to position
H
(bright
field).
☞
The 0.9/1.25 H condenser (000000-1104-285) can be used for transmitted-light bright field
without the slider.
Содержание Axioskop 40
Страница 1: ...Operating Manual Axioskop 40 Axioskop 40 FL Routine microscope...
Страница 14: ...INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0 14 B 40 810 e 12 01...
Страница 30: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1 16 B 40 810 e 12 01...
Страница 77: ...Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40 810 e 12 01 3 3...
Страница 128: ...OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3 54 B 40 810 e 12 01...
Страница 138: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 40 Carl Zeiss Axioskop 40 FL 4 10 B 40 810 e 12 01...
Страница 146: ...APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL A 8 B 40 810 e 12 01...