Zeiss Axioskop 40, Руководство по эксплуатации

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Axioskop 40 INTRODUCTION
Axioskop 40 FL Microscopy in a few steps ... Carl Zeiss
B 40-810 e 12/01 0-7
Microscopy in a few steps using the example of "Setting of transmitted-light brightfield
according to KÖHLER"
☞
Please read the instructions regarding instrument safety and the Instrument Description
(Chapter 1) and Start-Up (Chapter 2) chapters before using the Axioskop 40 or Axioskop 40 FL
microscopes for the first time.
(1) As described in Chapter 2 (Start-Up), the microscope is ready for operation and switched on
according to Section 3.4.
(2) Place a high-contrast specimen on the mechanical stage (cover slip pointing up).
(3) Swivel in 10x objective on the nosepiece and focus on the specimen; where possible, always
move the specimen away from the objective!
(4) Move the condenser - e.g. condenser 0.9/1.25 H - to the upper stop position via the
condenser drive. The height adjustment must be preset in such a manner that the specimen is
not moved by the condenser. If the 0.9/1.25 H condenser is equipped with the slider for
PH/H/Var, place it in the middle position for bright field.
(5) Close the luminous-field diaphragm until it is visible in the field of view, even if not in focus
(Fig. 0-1/
A ).
(6) Lower condenser 0.9/1.25 H until the edge of the luminous-field diaphragm appears in focus
(Fig. 0-1/
B ).
(7) Center the luminous-field diaphragm using centering screws on the condenser carrier (Fig. 0-
1/
C ) and open it until its edge just disappears behind the field of view (Fig. 0-1/ D ).
(8) To set the aperture diaphragm (contrast), remove one eyepiece from the binocular tube and
set the aperture diaphragm to approx. 2/3 of the diameter of the objective exit pupil
(Fig. 0-1/
E ). Optimum contrast setting is dependent on the specimen used.
(9) Insert the eyepiece again and, where necessary, refocus on the specimen via the fine drive.
(10) After each objective change, the field size and objective aperture change, which makes it
necessary to repeat steps (5) to (9).
Fig. 0-1 Diaphragm setting in transmitted-
light bright field according to
KÖHLER