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OPERATION
Axioskop 40
Carl Zeiss
Illumination and contrast procedures
Axioskop 40 FL
3-32
B 40-810 e 12/01
(3)
Setting the Axioskop 40 and Axioskop 40 FL microscopes
•
Set the microscope as for transmitted-light bright field (refer to Section 3.5.1 (3)), taking care to
ensure that the interpupillary distance of the binocular tube has been set correctly (refer to Section
2.1.3).
•
Center the rotary mechanical stage (3-25/
3
) (refer to Section 2.2.9 (2)).
•
Swivel polarizer (3-25/
2
) into the beam path and position it to 0°, provided that a rotary polarizer is
used.
•
Swivel in the analyzer module on reflector turret (3-25/
1
). The field of view now appears dark on
account of the crossed polarizers.
•
Place Pol adjusting specimen on the microscope stage and turn the stage until the adjusting specimen
is in the dark position.
•
Switch off analyzer and align crosslines with the fissures of the specimen.
•
Then switch on the analyzer again and remove the adjusting specimen. The transmission directions of
the polarizer and analyzer are now parallel to the crosslines (polarizer EW, analyzer NS).
•
Turn the mechanical stage with specimen, e.g. an artificial fiber, until the specimen displays maximum
darkness. The fiber is now parallel to one of the two directions of the crosslines. If pronounced
deviations (5° and more) occur, a polarization microscope must be used.
☞
Do not change the interpupillary distance of the binocular tube now, since otherwise the angle
position of the crosslines with reference to the fiber will be changed.
•
Turn the stage by approximately 45° until the
longitudinal axis of the fiber is oriented in a NE-
SW direction (3-26). The specimen now
features optimum brightness (diagonal
position). In this position, the specimen may
display any color.
•
Push in compensator
λ
(only in Axioskop 40 FL
because the opening is otherwise occupied by
the analyzer slider).
Like the specimen, compensator
λ
is a birefringent
object, though with a defined path difference of
550 nm and the greatest vibration direction n
γ
defined to be oriented in a NE-SW direction.
The specimen changes its color when
compensator
λ
is pushed in. The type of color
change depends on the specimen’s orientation
(NE-SW or NW-SE).
The changes in color are caused by optical
interference. Here, the interference colors (path
differences) in both diagonal positions (NE-SW and
NW-SE) of the specimen must be compared.
Fig. 3-27
Diagram of the Michel-Lévy color
charts
Содержание Axioskop 40
Страница 1: ...Operating Manual Axioskop 40 Axioskop 40 FL Routine microscope...
Страница 14: ...INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0 14 B 40 810 e 12 01...
Страница 30: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1 16 B 40 810 e 12 01...
Страница 77: ...Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40 810 e 12 01 3 3...
Страница 128: ...OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3 54 B 40 810 e 12 01...
Страница 138: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 40 Carl Zeiss Axioskop 40 FL 4 10 B 40 810 e 12 01...
Страница 146: ...APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL A 8 B 40 810 e 12 01...