Zeiss Axioskop 40, Руководство по эксплуатации

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Axioskop 40 OPERATION
Axioskop 40 FL Illumination and contrast procedures Carl Zeiss
B 40-810 e 12/01 3-29
3.5.5 Setting transmitted-light oblique illumination
(1) General principle
Oblique illumination offers an additional opportunity to display a relief-like structure of specimens.
Oblique illumination allows light into the objective from one side only, which results in a relief-like image.
(2) Axioskop 40 and Axioskop 40 FL configuration for oblique illumination
−
0.9/1.25 H condenser with slider, equipped preferably with phase stops Ph 1 / Ph 2 or Ph 1 / Ph 3
with stop Ph 1 in adjacent slider position to the soft stop notches.
The procedure of oblique illumination would constitute a worsening in comparison with the VAREL
contrast therefore the application is not recommended in connection with sliders equipped for VAREL
contrast.
(3) Setting oblique illumination on the
Axioskop 40 and Axioskop 40 FL
•
Place slider (3-24/ 1 ) for phase stops on the
0.9/1.25 H condenser (3-24/ 2 ) into one of the
weaker stop positions (to the side of the middle
stop position).
•
Open luminous-field diaphragm completely.
•
Open aperture diaphragm (3-24/ 3 ) completely.
With a different slider equipment to the one
recommended incident light through an
adjacent stop with a larger opening is possible
in the weaker stops. In such cases close the
aperture diaphragm until you obtain an
optimum image.
Fig. 3-24 Condenser 0.9/1.25 H and slider
with phase stops