Axioskop 40
OPERATION
Axioskop 40 FL
Illumination and contrast procedures
Carl Zeiss
B 40-810 e 12/01
3-35
•
Switch on the HBO 103 or HBO 50 mercury
vapor short-arc lamp (3-28/
2
) and allow it to
warm up to operation temperature for some 15
minutes.
•
Select the FL P&C reflector module in the
reflector turret (3-28/
1
) containing the required
fluorescence filter combination (depending on
excitation) and switch it on.
•
When using the incident-light filter slider
(3-28/
4
), unblock the light path in the incident-
light illuminator.
•
Remove one eyepiece from the tube and set
the aperture diaphragm (3-28/
3
) by opening it
until the entire objective exit pupil is unblocked.
•
Insert the eyepiece in the tube again and close
luminous-field diaphragm (3-28/
5
) until it is
visible in the field of view.
•
Use the two centering screws (3-28/
6
to center
the luminous-field diaphragm in relation to the
edge of the field of view.
•
Either open the luminous-field diaphragm until
it just disappears behind the edge of the field of
view or, if there is a risk of specimen bleaching,
reduce it until it is visible in the field of view.
•
Finally, refocus on the specimen and optimize
the HBO 103 collector position as described in
Section 2.1.12. Set the collector in such a
manner that homogeneous illumination is
obtained with the short-wave excitation
reflector module. When modules with long-
wave excitation are used, correction of the
collector position is not required.
Fig. 3-28
Epi-fluorescence components of the
Axioskop 40 FL
Содержание Axioskop 40
Страница 1: ...Operating Manual Axioskop 40 Axioskop 40 FL Routine microscope...
Страница 14: ...INTRODUCTION Axioskop 40 Carl Zeiss Axioskop 40 FL 0 14 B 40 810 e 12 01...
Страница 30: ...INSTRUMENT DESCRIPTION Axioskop 40 Carl Zeiss Axioskop 40 FL 1 16 B 40 810 e 12 01...
Страница 77: ...Axioskop 40 OPERATION Axioskop 40 FL Carl Zeiss B 40 810 e 12 01 3 3...
Страница 128: ...OPERATION Axioskop 40 Carl Zeiss Axioskop 40 FL 3 54 B 40 810 e 12 01...
Страница 138: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 40 Carl Zeiss Axioskop 40 FL 4 10 B 40 810 e 12 01...
Страница 146: ...APPENDIX Axioskop 40 Carl Zeiss Axioskop 40 FL A 8 B 40 810 e 12 01...