13
Basic Concentration Measurements—Basic A-%T-C
Setting the Wavelength and Mode
58
Orion AquaMate 7000 Vis and Orion AquaMate 8000 UV-Vis
Thermo Scientific
The steps for taking measurements in the two modes are similar—the only difference is
whether you measure a standard or enter a factor.
Setting the Wavelength and Mode
To set the wavelength and mode
1. Press
Set nm
or any other number key to set the wavelength.
2. Enter the wavelength for taking measurements and press
Set nm
again.
3. Press
Change Mode
until the appropriate measurement mode (Concentration with
Standard or Concentration with Factor) appears.
Using Conc/Std to Measure Concentration
In this mode a standard sample of well known concentration is used to determine the
concentration of samples. The concentration of unknown samples is determined
ratiometrically from the measured standard. The measurement can be expressed
mathematically as
where the standard concentration is precisely known, the Abs/%T of the standard and the
Abs/%T of the unknown sample are measured, and the sample concentration is calculated.
To use Conc/Std to measure concentration
1. If necessary, press
Change Mode
to switch to the Concentration with Standard mode.
If a 6-Position Cell Changer is installed, place the blank in the B position, and the
standard in position 1.
Sample
Unknown
of
%T
/
Abs
Standard
of
%T
/
Abs
ion
Concentrat
Standard
ion
Concentrat
Sample
