Thermo Scientific Orion AquaMate 7000 Vis, Руководство пользователя

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Thermo Scientific Orion AquaMate 7000 Vis and Orion AquaMate 8000 UV-Vis 135
25
Calculations for Software
Calculation Calculation(s) Graphs
Standard Curves
Partial sums
Where:
x
1
= Concentration of i
th
standard
y
1
= Absorbance of i
th
standard
N = number of standards
Linear regression
(general case) A = A(c)
Where:
A = absorbance
c = concentration
A(c) is defined by an equation of the form:
A(c) = a
4
c
4
+a
3
c
3
+a
2
c
2
+a
1
c+a
0
Where:
a
0
= Y-axis intercept
a
1
...a
4
= coefficients
(The coefficients are computed using the least squares
method.)
i
x SX
 
i
y SY
 
2
i
x SXX
 
2
i
y SYY
 
i i
y x SXY
 
 
2
2
* SX SXX N x x SQX
i
    
 
2
2
* SY SYY N y y SQY
i
    
  
SY SX SXY N y y x x SSXY
i i
* *
2
     