3
Tuning and Calibrating Automatically in the ESI/MS Mode
Setting Up the Syringe Pump for Tuning and Calibration
Thermo Electron Corporation
LTQ XL Getting Started
45
Setting Up the Syringe
Pump for Tuning and
Calibration
You introduce tuning and calibration solution into the API source with a
syringe infusion pump. A syringe pump allows you to infuse a sample
solution into the API source for extended periods of time.
The syringe pump and syringe are located on the front panel of your LTQ
XL MS detector. To infuse solution for tuning and calibration, you install
on the pump a 500-mL Unimetrics® syringe containing the calibration
solution.
Set up the syringe pump for infusion
1. Connect a 4 cm (1.5 in.) segment of Teflon
®
tube with a (brown)
fingertight fitting and a (brown) ferrule to the (black) LC union. See
Figure 16.
Plumbing connections for the syringe
2. Load a clean, 500-
μ
L Unimetrics syringe with 450
μ
L of the calibration
solution. (Refer to
Appendix A: “Sample Formulations”
for
a procedure for making the calibration solution.)
3. Insert the syringe needle into the segment of Teflon tube.
4. Place the syringe into the syringe holder of the syringe pump.
5. While squeezing the blue release button on the syringe pump handle,
push the handle forward until it just contacts the syringe plunger.
6. Connect a fused-silica infusion line from the LC union to the (stainless
steel) grounding union as follows. See
.
Note
To minimize the possibility of cross-contamination, use a different
syringe and section of fused silica tubing for the calibration solution than
you do for your sample solution.
LC Union
(P/N 00101-18202)
Ferrule
(P/N 00101-18196)
Teflon Tube
(P/N 00301-22803)
Fingertight fitting
(P/N 00101-18081)
