4
Tuning with Your Analyte in LC/ESI/MS Mode
Setting Up to Tune the MS Detector with Your Analyte
Thermo Electron Corporation
LTQ XL Getting Started
75
4. Define the scan parameters for tuning with your analyte in the ESI/MS
mode, as follows:
a. In the Instrument Control toolbar, click
Define Scan
to open the
Define Scan dialog box. See
.
b. In the Scan Description group box, in the Mass Range list box,
select
Normal
to allow for a selection of mass ranges between
m/z
150 to 2000.
c. In the Scan Rate list box, select
Normal
to specify a normal scan rate.
d. In the Scan Type list box, select
SIM
specify a Selected Ion
Monitoring experiment.
e. In the Scan Time group box, in the Number of Microscans spin box,
type
1
to set the total number of microscans to 1.
f. In the Max. Inject Time spin box, type
200.000
to specify a 200 ms
maximum injection time.
g. In the Scan Ranges group box, in the Input list box, select
Center/Width to make available the Center Mass and Width text
boxes in the Scan Ranges table.
h. In the Source Fragmentation group box, confirm that the On check
box is not selected (
) to specify that the ion source fragmentation
option is turned off.
i.
In the Scan Ranges group box (Scan Ranges table), in the Center
Mass text box, type the mass of your analyte to set the center of mass
for the scan range. If reserpine is the analyte you would enter
609.20
to set the center mass for the scan range to
m/z
609.20. If you are
using another analyte enter its mass value into the Center Mass text
box (
)
j.
In the Width text box, type
2.0
to set the width of the scan range to
2.0 daltons.
k. Verify that the settings in your Define Scan dialog box are the same
as those shown in
.
l.
Click
OK
to apply the MS detector scan parameters and to close the
Define Scan dialog box.
