LISST-Holo2
User’s Guide
7
2
Overview
—Using the LISST-Holo2
This is a very brief overview of LISST-Holo2 operation. For a more detailed introduction to the instrument, proceed
to section 3, Getting Started.
Unpack
Open shipping case and remove the LISST-Holo2, white plastic stands, Wi-Fi router,
blue 8-pin Ethernet cable, 5-pin Power and Communications Cable and External Power
Supply.
Make Connections
•
Connect the blue 8-Pin Ethernet cable to the LISST-Holo2 and then a port labeled 1
through 4. Do not use the yellow Internet port. If desired use the yellow Ethernet
cable and adapter to extend the length of the blue cable.
•
Connect the power to the router and power it up. It will take a couple minutes to
boot.
•
Connect the 5-pin Power and Communications cable to the 5-pin connector on the
LISST-Holo2 which is just below the serial number. This cable will only be used for
providing external power. The USB connection is normally not used.
•
Connect the External Power Supply to the receptacle in the cable.
Wake the LISST-Holo2
Move the white plastic lever on the Connector endcap from the “0” position to the “1”
position
and then quickly back to the “0” position. The end cap LED should start flashing
yellow, indicating that the LISST is booting. After about a minute, the LED should
change to green and flash once every 5 seconds, indicating the LISST is ready.
Connect Wirelessly
Using a WiFi-capable computer or tablet, connect to the wireless network named
LISST-
Holo2
using a password of
manyholograms
. Open a web browser and navigate to
. The LISST-Holo2 interface should appear in the browser. (Note
that this is the factory-default IP address, and can be changed.)
Set up Sampling
The LISST-Holo2 can be set up with 4 different sampling programs. Pressing the Home
tab on the top of the page will display a summary of each sampling program. Click on
the
hyperlink of the program labeled “Selected” to change its parameters. For testing
purposes, select
•
S
tart condition “Magnetic switch is in the 1 position”
•
Fixed Rate Mode with a sample interval of 1 second, and
•
S
top condition “Magnetic switch is in the 0 position.”
•
Click “Save & return to summary”
For more details on sampling programs, see section 5.3.
Collect Holograms
To start sampling, move the white endcap switch to
the “1” position. Data collection will
continue until you move the switch lever back
to the “0” position.
Offload Holograms
The holograms must be offloaded from the internal solid-state drive for processing.
Individual Holograms can be offloaded from the Images menu on the LISST-Holo2 User
Interface. To offload multiple holograms use an FTP utility like Filezilla (
). Offload the files into a separate directory if batch processing is to be used.
For more details, see section 5.7.
Batch Processing of
Holograms
Open the Holo Batch program and select File Paths for the Raw Holograms,
Reconstructed Images and the Size Distributions. Press the Process button to process
all the holograms in the selected folder. For details on Batch processing and installing
the Holo Batch program see Sections 4.2 and 4.4
Detailed Processing
of Holograms
Run the Holo Detail program to investigate individual holograms in more details than the
Holo Batch program. Use the Hologram Content Rating to select a hologram of interest.
Select a Region of Interest (ROI) and click on the Reconstruct button. Use the slider to
adjust the depth of the reconstructed image. For more information on Holo Detail, see
Section 4.5.
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