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LISST-Holo2
User’s Guide
Advanced Settings
Additional reconstruction options are available under Options ->
Advanced Settings.
The only parameters that should be adjusted are the index of refraction
and number of images in rolling background subtraction. An average
value of 1.333 for water is set as the default. This should be changed if
the hologram was taken in air or in any other substance with a different
index of refraction.
The number of images included in rolling background subtraction is the
number of images to average together and subtract from a hologram
when computing the Hologram Content Rating (discussed later). This is
a rolling background subtraction. For example, the default value is 10,
therefore, the holograms averaged together would be +/- 5 holograms
from the current hologram.
The Offset, Pixel Size, and Wavelength are fixed for the instrument and
should not be adjusted.
Set ROI and Reconstruct Buttons
Select the size of the ROI using the radio buttons below the hologram.
After selecting the ROI size press the
‘Set ROI’ button.
The ROI box will appear as a blue square overlaid on the hologram. The
ROI can be moved to another location by moving the mouse over the
ROI, selecting it, and then moving it around to the desired location within
the hologram.
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