LISST-Holo2
User’s Guide
13
4
Hologram Processing with Holo Batch and Holo Detail
4.1 Intro
The holograms are processed using two MATLAB-based programs called Holo Detail and Holo
Batch.
You do not need to be connected to the LISST-Holo2 to process previously offloaded holograms.
4.2 Install the hologram
processing software
To install the software, plug in the USB memory card that was shipped with
your instrument and locate the LISST-Holo2 software folder. Or visit
and download the software package from the LISST-
Holo2 page. Our software supports 64-bit Windows and Mac OS X operating
systems. You must select the software that matches your machine.
Inside the software folder you will see two folders and one application. The
folders contain the Holo Batch and Holo Detail applications. However, before
these programs can be run, the Matlab Compiler Runtime (MCR) must be
installed. The installer for the MCR is included in the software package and
has
a name such as ‘MCR_R2017a_win64_installer.exe’.
Double click the MCR installer. It will start by extracting files, which may take
several minutes. (Note that the installation may appear slightly different on
Mac OS X)
After the files are extracted the installer will open. Follow the onscreen
instructions to install the MCR. The installation may take a significant amount
of time depending on your machine.
The Holo Batch and Holo Detail applications do not need to be installed on
your computer. The .exe file contained in each folder can be run as a
standalone application. You can move the Holo Batch and Holo Detail folders
to a location of your choosing (for example into the Programs folder). It may
also be helpful to create a shortcut of the batch and detail applications and
place them on the desktop.
Should you like to add the program to the taskbar or start menu, you can right
click on the program icon and select ‘Pin to Taskbar’ or ‘Pin to Start Menu.’
4.3 Process holograms using
Holo Batch and Holo Detail
Two different programs are supplied for processing holograms; Holo Batch
and Holo Detail.
Holo Batch preforms batch processing of holograms, and automatically
outputs the size distribution as well as composite images showing the in-focus
particles.
Holo Detail is used to sort holograms or perform individual hologram
reconstructions. Holo Detail is can be a good way to sort holograms before
processing them with Holo Batch.
In the following sections, the use of these two programs will be explained.
Videos describing the use of both Holo Batch and Holo Detail are available on
the LISST-Holo2 webpage (
www.SequoiaSci.com/LISST-Holo2-videos
Содержание LISST-Holo2
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