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LISST-Holo2 

User’s Guide          

55 

12  Warranty 

 

STATEMENT OF LIMITED WARRANTY AND LIABILITY 
 

This Statement of Limited Warranty applies to all Sequoia Scientific, Inc. (“SEQUOIA”) products ("Products"). Any additional or 
different terms, including any terms in any purchase order, will be of no effect unless agreed to in writing by an authorized 
representative of SEQUOIA as reflected in a written SEQUOIA quotation.  
 
1. Limited Warranty 
SEQUOIA warrants that upon delivery by SEQUOIA (a) the Products will be free from defects in materials and workmanship, 
(b) the Products will perform substantially in accordance with SEQUOIA's applicable specifications, and (c) any Products (or 
components or parts thereof) that are manufactured by SEQUOIA do not infringe any U.S. patent or copyright. 
 
2. Correction of Non-Compliance 
If,  during the twelve mon

ths after delivery (the “Warranty Period”), any Product does not comply with the warranties set forth in 

1(a) and 1(b) above, SEQUOIA will, at its option, either (a) repair the Product, (b) replace the Product, or (c) refund the 
purchase price paid by Customer to SEQUOIA for the Product; provided that Customer gives SEQUOIA written notice of the 
noncompliance within the Warranty Period and ships the Product to SEQUOIA within one month after the end of the Warranty 
Period.  As to any Product repaired or replaced by SEQUOIA, the Warranty Period will end upon the later of the end of the 
original Warranty Period or 90 days after SEQUOIA's delivery of the repaired or replacement Product to Customer. Any 
Product, component, part or other item replaced by SEQUOIA becomes the property of SEQUOIA.  SEQUOIA may use 
refurbished components in the repair of Products supplied hereunder.  
 
SEQUOIA's warranties shall be void and not apply if the Product has been subjected to  misuse or alteration or repaired by a 
party not approved by SEQUOIA or the serial number on a product (if applicable) has been altered or defaced.  SEQUOIA shall 
not be liable for normal wear and tear (such as replacement of consumables), nor for defects or failure caused by maintenance, 
misuse, negligence or failure resulting from non-

compliance with SEQUOIA’s specifications, operating or maintenance 

manuals. 
 
3. Infringement Indemnification 
If any Product does not comply with the warranty set forth in 1(c) above, SEQUOIA will defend and indemnify Customer against 
any third-party claim asserted in any proceeding against Customer based on this noncompliance; provided that Customer gives 
SEQUOIA prompt written notice of the claim, SEQUOIA has exclusive control over the defense and settlement of the claim, 
Customer provides such assistance as SEQUOIA may request in connection with the defense and settlement of the claim (in 
which event SEQUOIA will reimburse the reasonable out-of-pocket costs incurred by Customer to provide such assistance), 
Customer does not settle the claim without the prior written consent of SEQUOIA and, upon SEQUOIA's request, Customer 
returns the Non-Complying Product to SEQUOIA for modification, replacement or a refund of the purchase price paid by 
Customer to SEQUOIA for the Non-Complying Product, less a reasonable allowance for Customer's use prior to return. 
 
4. Exclusive Warranties 
THE WARRANTIES SET FORTH IN PARAGRAPH 1 ABOVE ARE EXCLUSIVE AND IN LIEU OF ALL OTHER 
WARRANTIES, EXPRESS OR IMPLIED. SEQUOIA DISCLAIMS ANY AND ALL WARRANTIES, EXPRESS OR IMPLIED 
(INCLUDING, BUT NOT LIMITED TO, ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A 
PARTICULAR PURPOSE, AND ANY IMPLIED WARRANTY ARISING FROM COURSE OF PERFORMANCE, COURSE OF 
DEALING, OR USAGE OF TRADE) OTHER THAN THOSE SPECIFICALLY SET FORTH IN PARAGRAPH 1. 
 
5. Exclusive Remedies 
CUSTOMER'S RIGHTS AND REMEDIES SET FORTH IN PARAGRAPHS 2 AND 3 ABOVE ARE EXCLUSIVE AND IN LIEU 
OF ANY AND ALL OTHER RIGHTS AND REMEDIES FOR ANY BREACH OF OR OTHER FAILURE TO COMPLY WITH ANY 
WARRANTY WITH REGARD TO ANY PRODUCT. 
 
6. No Consequential Damages 
SEQUOIA will not be liable for any indirect, incidental, special or consequential damages, any cover, or any loss of revenue, 
profit, data or use. 
 
7. Limitations of Liability 
SEQUOIA's liability (whether in contract, tort, or otherwise; and notwithstanding any fault, negligence, strict liability or product 
liability) with regard to any Product (including, but not limited to, any breach of or default by SEQUOIA) will in no event exceed 
the purchase price paid by Customer to SEQUOIA for such Product. Further, SEQUOIA will not be liable for, or be in breach of 
or default on account of, any delay or failure to perform as a result of any cause, condition or circumstance beyond SEQUOIA's 
reasonable control. 
 
8. Indemnification by Customer 
Customer acknowledges that the Products are designed and manufactured for use in non-critical, monitoring situations.  If 
Customer chooses to purchase a Product or Products for use in applications that could result in damages in excess of the price 
of the Product if the Product does not operate properly or otherwise fails, Customer acknowledges and agrees that it is 

Содержание LISST-Holo2

Страница 1: ...equoia Scientific Inc 2700 Richards Road Suite 107 Bellevue WA 98005 USA Tel 1 425 641 0944 Fax 1 425 643 0595 info SequoiaSci com www SequoiaSci com LISST Holo2 User s Manual Version 1 0 January 2018...

Страница 2: ......

Страница 3: ...cal distributor a list of our international distributors can be found on www SequoiaSci com or Sequoia Scientific Inc directly Please be sure to include the instrument serial number with any correspon...

Страница 4: ...ection 35 5 6 Stop Data Collection 35 5 7 Downloading Raw Holograms 35 5 8 Viewing raw holograms in OpenSeeIt 37 5 9 Next Deployment or Powering Down 39 6 Reviewing Instrument Info and Other Settings...

Страница 5: ...11 7 5 Pin Bulkhead Connector Pin Assignments 53 11 8 6 Pin Bulkhead Connector Pin Assignments 54 12 Warranty 55 13 Index 57...

Страница 6: ...Thus from one hologram 100 in focus photographic frames are produced and particles appear in sharp focus at the plane where they existed The reconstruction software is provided Holography is data inte...

Страница 7: ...t IP address and can be changed Set up Sampling The LISST Holo2 can be set up with 4 different sampling programs Pressing the Home tab on the top of the page will display a summary of each sampling pr...

Страница 8: ...on the stands Take out the clear plastic box and open it up Inside you will find a few cables and power supplies described below The blue cable is an Ethernet cable used for communication with the LI...

Страница 9: ...port Use one of the other four available ports Connect the router power cable to the router Plug the router power supply into a 110 220VAC power source When the router completes its booting procedure...

Страница 10: ...ght indicators NOTE When external power is provided the LISST Holo2 will run off external power instead of the internal batteries If external power is disconnected the LISST Holo2 will automatically s...

Страница 11: ...s need to save it to a folder on your PC pgm format Holograms are stored in pgm format which is a lossless image format To see the holograms on your PC you may need to download and install OpenSeeIt h...

Страница 12: ...gap Center the openings of the chamber on the windows and insert the U shaped spacer so that the O rings seal on the area around the window It can be helpful to push the sliding part against the windo...

Страница 13: ...acted the installer will open Follow the onscreen instructions to install the MCR The installation may take a significant amount of time depending on your machine The Holo Batch and Holo Detail applic...

Страница 14: ...struction Parameters Select Output Options Select Particle Bin Size Select Paths Folders Select Files The Holo Batch is shipped with factory default settings for the Reconstruction Parameters and thes...

Страница 15: ...overlapping ROI s with a size of 1024 1024 pixels are chosen Particle counts will then be obtained from both ROIs and adjusted so that particles in the overlapping parts are only counted once If you c...

Страница 16: ...th is 658 nm The reconstruction software needs this value in order to properly reconstruct the holograms DO NOT CHANGE THIS VALUE The Index of Refraction is for the medium in which the LISST HOLO2 is...

Страница 17: ...s A csv file containing the size distribution for each hologram is written by default even if none of the check boxes or the right are selected During hologram reconstruction an image containing both...

Страница 18: ...omputer with at least 6 GB of RAM and are using a reconstruction step size of 0 5 mm or more If this option is selected a warning message will remind you of the requirements of running the higher memo...

Страница 19: ...Finally click the Size Distributions button to select the folder where you want the particle size distributions to be stored If you have selected to output CSV or MAT files this is where they will be...

Страница 20: ...nd files which will be averaged into one background file before subtracting It is not necessary to supply a background hologram in order to process your holograms if you do not select a background non...

Страница 21: ...sing to exit If the data visualization option is selected the plots shown at the left will be updated after each hologram is processed A status bar will be displayed at the bottom of the screen showin...

Страница 22: ...t window blue particles very close to the receive window Using a pair of red and blue 3 D glasses will display the particles with a 3 D effect where the red particles will appear closer to the viewer...

Страница 23: ...ened up in MATLAB It contains a structure called PartStats It has dimensions N 17 where N is the number of particles detected in the ROI The 10 columns have the following information about each partic...

Страница 24: ...s particles that appear on that particular slice The distance from the transmit window of each slice is given in the image filename For example the image on the left is 016 0270 z22 500 TIFF meaning i...

Страница 25: ...o select a folder for browsing your holograms Select the folder where your holograms are stored then click OK If you are just getting stated navigate to the Example Holograms folder located on the shi...

Страница 26: ...ether and subtract from a hologram when computing the Hologram Content Rating discussed later This is a rolling background subtraction For example the default value is 10 therefore the holograms avera...

Страница 27: ...t button to initiate the reconstruction process Upon reconstruction the individual slices can be viewed on the right by clicking on the left and right arrows The X Y and Z axes of the reconstructed im...

Страница 28: ...click to mark the end of the transect a figure similar to the one on the left will pop up showing the pixel intensities along the selected transect Zoom and Reset Buttons Clicking the zoom button enab...

Страница 29: ...tures for sorting holograms based on time stamp depth or content After a long deployment that has generated thousands of holograms this can be useful tool to sort or organize holograms before processi...

Страница 30: ...w view and reconstruct these holograms as described in the previous section Holo Detail also allows you view plots of the hologram meta data Switching to Plot View under the Data View Panel allows you...

Страница 31: ...r the batteries to charge The vent is the white plastic tab on the LISST Holo2 endcap Unscrew the vent until the red O ring is clearly visible as shown on the left Do not open the vent past the point...

Страница 32: ...but fast charging still doesn t start also connect the external power supply through the 5 pin bulkhead connector this is necessary when the batteries are so deeply discharged that the battery supervi...

Страница 33: ...switch is in the 1 position If it is past the specified time the sampling will start immediately if the magnetic switch is in the 1 position Be sure to check the homepage to make sure your LISST HOLO2...

Страница 34: ...e in GMT The Stop after setting will stop the sampling after the specified amount of time from the start of data collection The Depth value will stop when the depth is deeper than the specified depth...

Страница 35: ...move the switch to the 0 position Within a few seconds the LED on the endcap should change from Red to Green If the data collection has already stopped the LED will be blinking green regardless of the...

Страница 36: ...ndow To the left is the destination folder selection Navigate to the desired destination folder In this example we will offload to a folder on the C drive called HOLO Offload Note that the destination...

Страница 37: ...holograms straight across to the destination folder window on the left side of the screen The selected holograms will then transfer to the destination folder To DELETE the holograms right click on th...

Страница 38: ...follow the instructions below Open up OpenSeeIt and select Browser Mode from the View Menu Navigate to the folder where your holograms are stored They will all be displayed in the bottom left of the O...

Страница 39: ...the current sampling program For short term or long term storage of the LISST Holo2 it should be put into Sleep mode The instrument will automatically go into lower power sleep mode after 10 minutes...

Страница 40: ...user select which program should control the LISST HOLO2 sampling Up to 4 programs can be loaded in the LISST HOLO2 In the example to the left Program 1 is selected If another program is to be selecte...

Страница 41: ...cting Configure on the home page main menu will bring up a window listing many parameters and allows their values to be changed Values on this page should not be changed without detailed knowledge of...

Страница 42: ...ISST Holo2 goes to sleep you will lose connection with the instrument Click the Reboot button to reboot the LISST HOLO2 If you reboot you must re enter the LISST HOLO2 IP address in your browser windo...

Страница 43: ...dates the next time it reboots This requires connection to the Internet Use the Reboot button above when ready Network parameters Use Network parameters to change the IP address of the LISST HOLO2 Cli...

Страница 44: ...e top of the About page contains information about the hardware and firmware loaded in the instrument as well as the serial number of the instrument Below this section are a few FAQ s as well as links...

Страница 45: ...nce Make sure that the windows are as clean as possible The windows can be cleaned with Windex but often a mild lukewarm soap solution is the best for cleaning optical windows Dip a finger in the soap...

Страница 46: ...uter you need to enter the WAP PSK password which is manyholograms Then power up the LISST HOLO2 and initiate communication as described in section 3 2 Updating LISST HOLO2 software via the Internet T...

Страница 47: ...ke effect immediately so you will need to enter the new IP address in your browser to continue working with the LISST HOLO2 8 4 Recovering Lost IP Address Using the Serial Port In order to communicate...

Страница 48: ...LISST Holo2 is fully booted press the enter key several times You should see a response similar to the one on the left The error messages displayed in the terminal are normal and should be ignored The...

Страница 49: ...sables automatic sleep and click the Apply button Why can t the LISST Holo2 be used when the flow speed is more than 2 m s It takes 2 microseconds to capture the hologram using the digital camera The...

Страница 50: ...more thorough explanation The optional Path Reduction Module can be used to shorten the optical path which will allow up to a factor of 5 increase in the maximum concentration Mean Particle Diameter W...

Страница 51: ...rnet communication GREEN followed by BLUE every 2 seconds A sampling program is active but is waiting for the start condition to be met GREEN flashing continuously twice per second A sampling program...

Страница 52: ...ta processing yields in focus particle images and volume distribution Automated firmware updates possible when instrument is connected to the Internet 11 2 Parameters Measured and Derived Particle ima...

Страница 53: ...40 seconds when all systems are turning on 11 6 Dimension Drawing LISST Holo2 dimensions in inches mm 11 7 5 Pin Bulkhead Connector Pin Assignments Pin Function Teledyne Impulse part MCBH WB 5 MP SS M...

Страница 54: ...6 Pin Bulkhead Connector Pin Assignments Pin Function Teledyne Impulse part MBCH WB 6 MP SS MALE FACE VIEW 1 Power Ground 2 Battery Charge Input 3 Digital Output active low 4 Digital Input active high...

Страница 55: ...tomer based on this noncompliance provided that Customer gives SEQUOIA prompt written notice of the claim SEQUOIA has exclusive control over the defense and settlement of the claim Customer provides s...

Страница 56: ...erating Products Customer will not use any such software for any other purpose modify adapt translate or create derivative works based on any such software or disassemble decompile or reverse engineer...

Страница 57: ...truction parameters 14 ROI Region Of Interest 15 HOLO_Detail 25 Hologram 49 details 41 download from internal drive 35 41 enter comment 42 processing 13 view in OpenSeeIt 37 Home page 10 40 Images pag...

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