Sequoia LISST-Holo2, Руководство пользователя

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22 LISST-Holo2
User’s Guide
.TIFF files output by HOLO_Batch
If you selected to output dep/mon images, two .TIFF files will be
created for every hologram process. They will be placed in the
reconstructed images folder
The
–mon suffix image is a montage of all the in-focus particles
that were found in all the planes within an ROI.
The scale bar length can be adjusted prior to processing in the
Advanced section of Holo Batch.
Note that only the part of the hologram covered by the ROI is
displayed on the
–mon image. The rest of the hologram was not
analyzed and therefore cannot be displayed.
The
–dep suffix image is a color coded image, where the
particles within the ROI have been colored according to their
depth of focus.
As with the
–mon image, only the part of the hologram covered
by the ROI is displayed on the
–dep image.
The scale bar is all blue and displayed in the bottom left corner.
The color bar in the bottom right corner shows the location of the
particles relative to the transmit window. Red particles are very
close to the transmit window, blue particles very close to the
receive window.
Using a pair of red and blue 3-D glasses will display the particles
with a 3-D effect, where the red particles will appear closer to the
viewer than the blue particles.
.CSV files output by HOLO_Batch
A composite file with the name
firsthologram _ lasthologram _All.csv, where firsthologram and lasthologram are the
filenames of the first and last holograms that were selected for processing. This file is always generated for each run of
Holo Batch. This composite file has all the information about sample date and time, water depth, temperature, and the
size distribution (given as the volume concentration in (µl / l) per size bin).
Finally, the composite file has in its first row the lower limit of the size bin for the size distributions in columns 26:75. In
the 2
nd
row is the mid-point of the size bin for the size distributions in columns 26:75 and in the 3
rd
row is the upper limit of
the size bin for the size distributions. The table below shows the parameters in columns 1-75 of the .CSV file.
Column # Value Unit
1 Year (YYYY) N/A
2 Month (MM) N/A
3 Day (DD) N/A
4 Hour (HH) N/A
5 Minute (MM) N/A
6 Second (SS) N/A
7 1/100s of a second (hhh) NOTE: Currently not available. NaN (Not a Number) displayed
instead.