Sequoia LISST-Holo2, Руководство пользователя

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LISST-Holo2
User’s Guide
15
The Change ROIs button opens up a window for selecting the
ROI (Region Of Interest) to be processed.
The ROI is the part(s) of the hologram that will be reconstructed.
Particle counts will only be computed from the reconstructed part
of a hologram.
ROI’s must be defined as a square region to allow for correct
hologram reconstruction. Therefore, the rectangular image (1600
x 1200) cannot be treated as a single ROI.
Once selected, the ROI will be the same for all holograms.
In t
his example, 2 overlapping ROI’s with a size of 1024

1024
pixels are chosen. Particle counts will then be obtained from both
ROIs, and adjusted so that particles in the overlapping parts are
only counted once.
If you click the Add button, a 3
rd
ROI will be added, which you
can then drag to the desired location.
If you click the Delete button, the last added ROI will be deleted.
Reconstruction of holograms is memory demanding, so on PC’s
with smaller memory (<4GB), or slower processors (<2GHz), the
512

512 pixels ROI should be selected.
Note that you can delete and add ROI’s using the Delete and
Add buttons.
In this example to the left, one of the 1024

1024 pixel
ROI’s
have been deleted.
Subsequently the Delete button has been grayed out.