Roche Nimblegen MS 200, Руководство оператора

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NimbleGen MS 200 Microarray Scanner Operator’s Manual v1.0 | 43
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Far left = Fast/Low (24 lines per second (12Hz) parallel)
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Mid left = Accelerated/Standard (24 lines per second (12Hz)
sequential)
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Mid right = Medium/Advance (16 lines per second (8Hz) parallel)
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Far right = Slow/High (16 lines per second (8Hz) sequential)
When scanning in parallel mode, the Microarray Scanner collects data
for both channels at the same time. With sequential mode, it performs
two individual scans: one for each channel.
Channel. Select the laser channel (532 or 635nm).
Gain. Adjust the gain for the photomultiplier tubes (PMTs) from 0.01
to 1000% in small or large increments.
Laser intensity. Select laser intensity at 0.1, 1, 10, 25, 50, 75, or 100% for
each channel.
It is recommended to keep the laser intensity set to 100% and
adjust the PMT gain setting if necessary.
Autogain. Select this checkbox to activate the autogain procedure.
During the autogain procedure, the instrument software scans the
specified autogain area and equalizes the intensity values for the
channels.
Apply to slide number x and cycle x. Select this option to apply parameter
settings only to the selected slide and corresponding slide cycle.
Apply to slides with parameter set. Select this option to apply parameter
settings to all slide positions with the same parameter letter code in the
Magazine Control.
3. To save any changes to the settings, click Apply . Or, click Reset to
cancel changes and leave the parameter settings unchanged.
4.
If desired, click Save to save settings to a parameters file for future use.