Rotor-Gene Q MDx CE User Manual 02/2022
90
Rep. Ct. 95% C.I.:
A C
T
range that, statistically, accounts for 95% of the variation in the C
T
value. This
is a conservative statistical measure, which can be used as a quality measure. This
range can be narrowed by running more replicates or by having less variation in the
replicates.
Rep. Calc. Conc.:
The calculated concentration for all the samples with the same name.
Note
: This is not the simple average of the calculated concentrations. It is the
geometric mean, which is a mathematically more appropriate average due to the
exponential nature of real-time amplification.
Rep. Calc. Conc. 95% C.I.:
A range of concentrations that accounts for 95% of the variation in the individual
sample as well as the linear regression model on which it is based. An interpretation
of this measure is that it is the range of concentrations that could be expected 95%
of the time if this run were performed repeatedly with the same amount of variation.
This is a conservative estimate, and the range can be quite large due to the variation
inherent in any real-time analysis. This range can be large if standards are run with
concentrations different from the unknown samples, if a small number of replicates
are used, or if there is significant variation.
Important
: The variations reported by this measure are inherent to the exponential
process of real-time amplification and are not due to the Rotor-Gene Q MDx. Similar
tests performed on block-based cyclers would yield greater variation due to the lower
temperature uniformity of block-based systems. To compare cyclers if desired, we
recommend comparing the standard deviation of the CT value.
Note
: More detailed information on confidence intervals is available in Appendix B.
Note
: Except for Color, Name, Ct, and Ct Comment, each of the columns can be displayed or
hidden by right-clicking on the window and then selecting or deselecting the column name.
For increased convenience, the
AutoStat
feature automatically calculates the average, standard
deviation, and minimum and maximum values of samples of interest. Select the results of interest by