Rotor-Gene Q MDx CE User Manual 02/2022
184
11.1
Log Archives
The software keeps an unmodified record of each run, along with diagnostic information, in its Log
Archive repository. By using the
Help
,
Send Support Email
option, you can send an e-mail along
with all the necessary diagnostic information to QIAGEN Technical Services (see Section 6.12.1).
To save disk space, only Log Archives of the 60 most recent runs are stored. Older run Log Archives
will be overwritten as new run Log Archives are created.
11.2
Hardware and software errors
11.2.1
HRM troubleshooting
Comments and suggestions
Unable to run HRM
Rotor-Gene Q MDx model is not
HRM equipped
Contact your local QIAGEN representative.
No HRM data obtained
Incorrect setup
Check filter settings.
Check if the rotor type is correct.
Check if the correct reagents have been used.
Check if the reaction was set up correctly.
Run a positive control experiment (i.e., an assay that is known to provide
results).
Plots look jagged
Poor or no amplification
Check if the correct protocols and reagents have been used. We
recommend QIAGEN kits for HRM analysis.
Check if the reaction was set up correctly.
Check the cycling conditions.
Check the starting quality and quantity of the template. We recommend
QIAGEN kits for sample preparation.
Amplification or melt plots are saturated
Gain set too high
Use
Auto-Gain Optimisation
(see page 61).
Confidence percentages have changed
Normalization regions were moved
by clicking and dragging
Only move normalization regions if it is necessary to avoid parts of the melt
curve.
Outliers are present in the data
Inconsistent reaction setup
Inhibitors present in sample
Too little or degraded template
Check if the correct reagents have been used.
Check that the tubes used are uniform.
Check that the same master mix was used for all samples.
Check the starting quality and quantity of the template.