Rotor-Gene Q MDx CE User Manual 02/2022
115
For example:
Sample
Type
Fluorescence
1
Positive control
53.6
2
Positive control
53.0
3
Negative control
4.5
4
Negative control
4.3
5
Sample
48.1
6
Sample
6.4
This run was a success, as the 2 positive and 2 negative controls are close together, and are outside
the fluorescence values of the samples.
The normalized values are:
Sample
Type
Expression (%)
1
Positive control
100.0
2
Positive control
97.3
3
Negative control
0.4
4
Negative control
0.0
5
Sample
84.2
6
Sample
4.0
Sample 1 was the positive control with the highest fluorescence, so it was set to 100%. The other
positive control was slightly lower. Sample 4, the lowest negative control was set to 0%. It is now
clear that sample 5 has probably amplified, whereas sample 6 has probably not amplified.
Note
: Depending on the positive and negative controls selected, it is possible to achieve expression
levels of greater than 100% or less than 0%. A result of greater than 100% can be interpreted to
mean that the sample is more highly expressed than the positive controls. A result of less than 0%
can be interpreted to mean that it is less likely that the sample amplified than that the negative
controls amplified. Since this analysis is qualitative, such results are not a concern.
If the negative controls result in higher fluorescence than the positive controls, the samples have
been incorrectly set up and the following message appears.