Rotor-Gene Q MDx CE User Manual 02/2022
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Set temperature to
:
Before reading, the Rotor-Gene Q MDx will be heated or cooled to match the
specified temperature. By default, this is set as the acquisition temperature.
Optimise All/Optimise Acquiring
:
Optimise All
will attempt to optimize all channels known by the software.
Optimise
Acquiring
will only optimize the channels that are used in the thermal profile defined
in the run (cycling and melt).
Perform Optimisation Before First
Acquisition
:
Check this box to perform Gain Optimisation at the first cycle in which data
acquisition occurs. This is recommended for Auto-Gain Optimisation.
Perform Optimisation At [x]
Degrees At Beginning of Run
:
Check this box to perform Gain Optimisation just before starting the run. The Rotor-
Gene Q MDx is heated to the specified temperature, Gain Optimisation is
performed, and then cycling begins on the first step, usually a Denature step. This
option may be chosen if a Gain Optimisation during the run would result in too much
time spent on the initial step. Usually
Perform Optimisation Before 1st Acquisition
is
preferred because Gain Optimisation is performed as close as possible to the run
conditions.
Channel Settings
:
This drop-down menu allows channels to be added. Choose the channel of interest
and click
Add
.
Edit
:
This opens a window in which the
Target Sample Range
can be set. The
Target
Sample Range
is the range of initial fluorescence that should be set for the sample in
the specified tube. Auto-Gain Optimisation reads each channel using gain settings
in the range specified by
Acceptable Gain Range
. It chooses the first gain setting that
results in a fluorescence reading within the
Target Sample Range
. In the example
shown, Auto-Gain Optimisation searches for a gain setting between –10 and 10
that gives a reading between 5 and 10 FI in tube 1. In general, for intercalating dyes
a
Target Sample Range
of 1–3 FI is appropriate, while a range of 5–10 FI is more
suitable for probe chemistries.