Rotor-Gene Q MDx CE User Manual 02/2022
53
Edit Profile
The
Edit Profile
window allows the cycling conditions and acquisition channels to be specified. The
initial profile shown is based on the template selected when setting up the run (see page 45). The
profile is displayed graphically. The list of the segments of the profile appears below the graphical
display. This list can include Hold (page 53), Cycling (page 54), Melt (page 54), or HRM if the
instrument has a HRM channel (page 57).
Each stage of the profile can be edited by clicking on the appropriate area of the graphical display
or on the name in the list, and then changing the settings which appear.
Insert after…:
This allows addition of a new cycle after the selected cycle.
Insert before…:
This allows addition of a new cycle before the selected cycle.
Remove:
This removes the selected cycle from the profile.
Hold
A Hold instructs the Rotor-Gene Q MDx to remain at the designated temperature for a set time. To
change the temperature, click on the
Hold Temperature
button and type or use the slide bar to select
the desired temperature. To change the duration of the Hold, click on the
Hold Time
,
mins
, and
secs
buttons.
If performing Optical Denature Cycling, a Hold can be used as a calibration step. In this case, a
calibration melt is performed before the Hold. By default, this is configured for the first Hold in the
run, but may be changed if required.