PerkinElmer FLEXAR SQ 300 MS, Руководство пользователя

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Starting Chromera. 31
12. After auto tune is complete, click on
Check Tune.
The
Status
light will turn
yellow
and
Status:
Executing check tune
displays. If a significant amount of time passes between the completion of
Auto Tune and the execution of Check Tune, click the “Sample Prime” button to get the calibration
mixture flowing again.
When check tune is complete a
Check Tune Results screen displays.
For each mass there are four evaluation criteria:
• The
Mass
criterion checks to see if the mass accuracy of a peak is /- 0.1 u of the
actual mass.
•
The Width criterion checks the Full Width at Half Maximum is between 0.5 and 0.7 u.
Note that the Width criteria is expanded up to 0.85 u for high mass ions.
• The
Area %
criterion checks the relative intensities of each mass in the tune relative the
most intense peak in the tuning mix.
•
The report also provides the Area counts for each mass after Check Tune is run.