PerkinElmer FLEXAR SQ 300 MS, Руководство пользователя

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Initial Process to Configure an Optimal Tune and Method on the SQ 300 MS Detector. 37
Setting up a Sample Infusion
Infusion is easy to accomplish using the SQ 300 MS Detector’s built in syringe pump. Typically, there are
two options for accomplishing this: infuse the standard at a low flow rate directly into the MS, or infuse it
into the LC stream running at a typical flow rate so as to also optimize “flow dependent” MS parameters
such as temperature and drying gas. The following example demonstrates how to infuse a reserpine
standard directly into the MS using a syringe. Reserpine was chosen because it is a widely used standard
in the LCMS community and it is readily available from a variety of commercial sources.
1. Fill a syringe with a ~100 pg/µl reserpine solution in LCMS grade methanol and water in a 3:1 ratio.
2. Disconnect the calibration line from the ESI sprayer on the SQ 300 MS and connect a Peek transfer
line from the syringe needle to the ESI sprayer.
3. With the syringe prepared for infusion, the next part of the process covered in the next section
involves creating a simple software evaluation routine to expedite the data evaluation process.
Syringe Pump
Disconnect
Capillary Tubing
to the Probe