PerkinElmer FLEXAR SQ 300 MS, Руководство пользователя

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Creating Methods and Sequences. 57
10. Select the most recent pos_1k tune file (with a date/time stamp), then click OK .
This example shows
pos_1k 29 Oct 2010
is selected as the most recent pos_1k tune file.
11. In the Global Variables section, click on Acquisition Function, select SIM from the drop-down list,
and then press Enter . This will convert the Tune from a scanning acquisition to a SIM acquisition.
12. To set the specific SIM mass, select Low m/z, type your value, and press Enter . This example
uses
m/
z
609.3
.
13. Set the
Pulse Counting Time (µs)
to
300000
.
This is a 300 msec dwell time, which will give excellent signal to noise. It is possible to acquire
data with much shorter dwell times to achieve higher sampling rates, such as those required for
UHPLC separations or if the acquisition requires monitoring many SIM ions “simultaneously”
(current limit is 38).
14. Set the
Drying Gas Flow Rate (L/min)
to
12
.
The optimal drying gas flow is dependent on a number of factors, such as the LC flow rate,
composition of the mobile phase and the temperature setting of the Drying Gas .
15. Click on
Capillary Exit (Volts)
and from the drop-down list and select
Single Valve
. Enter a
value of
150
.