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Chapter 3 Operation Details
32
Table 3.3-1 Selecting the microscopy method
Microscopy Revolving
nosepiece
Objective
Items required for the optical
path
BD changeover
lever
Remarks
BF
microscopy
under the
epi
illumination
5 revo.,
6 revo.,
7 revo.
•
For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
•
For 6 or 7 revo., BD
objective unavailable
⎯
BF (Press)
Normal bright-field microscopy under the
episcopic illumination.
DF
microscopy
under the
epi
illumination
5 revo. only BD objective only
⎯
DF (Pull)
Pull out BD field changeover lever to perform
dark-field microscopy under the episcopic
illumination.
Pull out BD field changeover lever to fully
auto-open field/aperture diaphragm (The dial
position remains the same).
Pull out BD field changeover lever to exclude
polarizer/analyzer unit or fluorescent unit when
it is in the optical path.
Simplified
polarization
microscopy
under the
epi
illumination
5 revo.,
6 revo.,
7 revo.
•
For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
•
For 6 or 7 revo., BD
objective unavailable
•
MA2-PA/MA2-UPA
polarizer/analyzer unit
•
For the sensitive color
polarization microscopy,
MA2-
λ
P
λ
plate available
BF (Press)
Set bright-field microscopy under the episcopic
illumination. And then, attach
polarizer/analyzer unit to make crossed Nicol’s
position.
Push
λ
plate into polarizer/analyzer unit and
place it into optical path.
DIC
microscopy
under the
epi
illumination
5 revo.,
6 revo.
LU EPI objectives or LU
BD objective only
•
For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
•
BD objective
unavailable for 6
revo.
For 5 revo.:
•
MA2-PA polarizer/analyzer
unit
•
L-DIHC/L-DIC slider (single
NR method)
•
For the sensitive color
polarization microscopy,
MA2-
λ
P
λ
plate available
For 6 revo.:
•
MA2-UPA polarizer/analyzer
unit
•
LV-DIHC/LV-DIC slider
(Senarmont method)
•
For the sensitive color
polarization microscopy,
MA2-
λ
P
λ
plate available
BF (Press)
Set bright-field microscopy under the episcopic
illumination. And then, attach
polarizer/analyzer unit to make crossed Nicol’s
position. Place DIC prism into optical path to
perform DIC microscopy.
To adjust the contrast, turn the prism setting
dial for the single NR method, whereas turn the
polarizer rotation ring for Senarmont method.
Push
λ
plate into polarizer/analyzer unit and
place it into optical path.
Epi-fl
microscopy
5 revo.,
6 revo.,
7 revo.
LU EPI objectives or LU
BD objective only
•
For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
•
For 6 or 7 revo., BD
objective unavailable
•
MA2-FL fluorescent unit
BF (Press)
Place a desired fluorescent unit G, B, BV or V
into optical path to perform epi-fl microscopy.
BF
microscopy
under the
dia
illumination
5 revo.,
6 revo.,
7 revo.
•
For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
•
For 6 or 7 revo., BD
objective unavailable
⎯
BF (Press)
Normal bright-field microscopy under the
diascopic illumination.
Simplified
polarization
microscopy
under the
dia
illumination
5 revo.,
6 revo.,
7 revo.
•
For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
•
For 6 or 7 revo., BD
objective unavailable
•
T-P2 polarizer
•
MA2-PA/MA2-UPA
polarizer/analyzer unit (for 6
revo., D-DA analyzer is also
available)
•
For the sensitive color
polarization microscopy,
TI-DIC
λ
plate available (for 6
revo., D-LP
λ
plate is also
available)
BF (Press)
Set bright-field microscopy under diascopic
illumination. And then, attach polarizer to
diascopic pillar illuminator and insert analyzer
into MA200 main body to make crossed Nicol’s
position. (For polarizer/analyzer unit, the
analyzer part is used.)
Attach TI-DIC
λ
plate to supporting pillar for
dia-Illuminator 100W. For 6 revo., D-LP
λ
plate
is also available.
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